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- 文献和实验
- 技术资料
- 级别:
详见MSDS文件
- 适应物种:
人,小鼠,大鼠,驴,其他,斑马鱼,细菌,鸡
- 保存条件:
-20°c
- 应用范围:
免疫荧光(IF)
- 抗体英文名:
β-Tubulin (9F3) Rabbit mAb (Alexa Fluor 555 Conjugate)
- 是否单克隆:
1
- 供应商:
CST
- 抗原:
/
- 库存:
大量
- 抗原来源:
/
- 保质期:
详见说明书
- 宿主:
兔
- 规格:
200 ul (100 assays)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> /200 ul (100 assays)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格: | 产品价格: | ¥面议 | |
|---|---|---|---|
| 规格: | 200 ul (100 assays) | 产品价格: | ¥请询价 |
| 规格: | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格: | ¥请询价 |
| 规格: | 200 ul (100 assays) | 产品价格: | ¥请询价 |
| 规格: | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格: | ¥请询价 |
Product Pathways - Cytoskeletal Signaling
β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116
PhosphoSitePlus ® protein, site, and accession data: TUBB
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IF-IC | H M R Mk Dm Z B (C) | Endogenous | Rabbit IgG |
Applications Key: IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey C=Chicken Dm=D. melanogaster Z=Zebrafish B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 2116:
- Immunofluorescence
Specificity / Sensitivity
β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) detects endogenous levels of total β-tubulin protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to the amino terminus of human β-tubulin. The antibody was conjugated to Alexa Fluor® 555 under optimal conditions with an F/P ratio of 2-6.
IF-IC
Confocal immunofluorescent analysis of HeLa cells, serum-starved (left) or 20% serum-treated (right), using β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) (red) and Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4854 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Description
Cell Signaling Technology antibody is conjugated to Alexa Fluor® 555 fluorescent dye and tested in-house for direct immunofluorescent analysis of human and monkey cells. The unconjugated antibody #2128 reacts with human, mouse, rat, monkey, bovine, zebrafish and fly β-tubulin protein. CST expects that β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) will also recognize β-tubulin in these species.
Background
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
- 2144 α-Tubulin Antibody
- 2125 α-Tubulin (11H10) Rabbit mAb
- 2146 β-Tubulin Antibody
- 2148 α/β-Tubulin Antibody
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.
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文献和实验CST 抗体 9 月特惠|购买任意 CST 抗体,+168 元即可入手 25μl 标签 / 内参抗体
HRP、Alexa Fluor® 488、Alexa Fluor® 594 等多种标记一抗可选。 热门可选产品参考 货号 产品名称 类型 加价购价格 S0B0005-25u1 B-actin Rabbit mAb (SDT-RO15) 裸抗 168 元 S0B0004-25u1 B-tubulin Recombinant Rabbit mAb (SDT-RO14) 裸抗 168 元 S0B0383-25u1 S-RMab® Myc tag Recombinant Rabbit
Recombinant Rabbit mAb (Alexa Fluor® 647 conjugate) (S-712-10) 二、抗独特型抗体(Anti-idiotype antibody) 抗独特型抗体更适合单一CAR 骨架的深度表征,它识别scFv可变区的专属独特表位,具有极强的靶点特异性,仅识别特定scFv序列构建的CAR,几乎不存在交叉反应。目前市面上已有多款成熟商业化抗独特型抗体,分别识别临床广泛使用的CAR scFv克隆,包括识别FMC63 scFv(CD19 CAR-T)、人源2C6 scFv
), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate)Secondary Antibody250µlIF-IC HCA4414SAnti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate)Secondary Antibody250µlIF-IC F4416SAnti-rat IgG (H+L), (Alexa Fluor® 488 Conjugate)Secondary
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