- 询价
- Cell Signaling Technology
- 2116
- USA
- 2026年01月13日
- 免疫荧光（IF）
- 兔
- 人,小鼠,大鼠,驴,其他,斑马鱼,细菌,鸡
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- 详细信息
- 文献和实验
- 技术资料
- 级别：
详见MSDS文件
- 适应物种：
人,小鼠,大鼠,驴,其他,斑马鱼,细菌,鸡
- 保存条件：
-20°c
- 应用范围：
免疫荧光（IF）
- 抗体英文名：
β-Tubulin (9F3) Rabbit mAb (Alexa Fluor 555 Conjugate)
- 是否单克隆：
1
- 供应商：
CST
- 抗原：
/
- 库存：
大量
- 抗原来源：
/
- 保质期：
详见说明书
- 宿主：
兔
- 规格：
200 ul (100 assays)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> /200 ul (100 assays)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥面议 | |
|---|---|---|---|
| 规格： | 200 ul (100 assays) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
| 规格： | 200 ul (100 assays) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Cytoskeletal Signaling
β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116
PhosphoSitePlus ® protein, site, and accession data: TUBB
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IF-IC | H M R Mk Dm Z B (C) | Endogenous | Rabbit IgG |
Applications Key: IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey C=Chicken Dm=D. melanogaster Z=Zebrafish B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 2116:
- Immunofluorescence
Specificity / Sensitivity
β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) detects endogenous levels of total β-tubulin protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to the amino terminus of human β-tubulin. The antibody was conjugated to Alexa Fluor® 555 under optimal conditions with an F/P ratio of 2-6.
IF-IC
Confocal immunofluorescent analysis of HeLa cells, serum-starved (left) or 20% serum-treated (right), using β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) (red) and Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4854 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Description
Cell Signaling Technology antibody is conjugated to Alexa Fluor® 555 fluorescent dye and tested in-house for direct immunofluorescent analysis of human and monkey cells. The unconjugated antibody #2128 reacts with human, mouse, rat, monkey, bovine, zebrafish and fly β-tubulin protein. CST expects that β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) will also recognize β-tubulin in these species.
Background
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
- 2144 α-Tubulin Antibody
- 2125 α-Tubulin (11H10) Rabbit mAb
- 2146 β-Tubulin Antibody
- 2148 α/β-Tubulin Antibody
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.
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文献和实验), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate)Secondary Antibody250µlIF-IC HCA4414SAnti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate)Secondary Antibody250µlIF-IC F4416SAnti-rat IgG (H+L), (Alexa Fluor® 488 Conjugate)Secondary
：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
High‐Throughput, Multiplexed Analysis of 3‐Nitrotyrosine in Individual Proteins
.1 ) Phosphate‐buffered saline (PBS; Fisher Scientific) Alexa Fluor 546–conjugated goat anti—rabbit IgG (Invitrogen, cat. no. A‐11035
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