EGR1 (44D5) Rabbit mAb

价格询价

品牌Cell Signaling Technology
货号
4154
产地USA
更新日期2026年10月11日

应用范围W, IP, IF-IC, F, ChIP
适应物种H,M,R,B

美国CST中国公司(丁香通商铺)

品牌商

13 年钻石会员

详细信息
文献和实验
技术资料

抗体英文名：
EGR1 (44D5) Rabbit mAb
抗原：
synthetic peptide corresponding to the amino terminus of the sequence of human EGR1
应用范围：
W, IP, IF-IC, F, ChIP
适应物种：
H,M,R,B
保质期：
详见说明书
库存：
大量
供应商：
CST
级别：
详见MSDS文件
是否单克隆：
1
保存条件：
-20°c
规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity

规格：		产品价格：	￥请询价
规格：	100 ul (10 western blots)	产品价格：	￥请询价
规格：	carrier free & custom formulation / quantity	产品价格：	￥请询价

pathway more info application references datasheet PDF MSDS PDF protocols

Applications Key: W=Western Blotting IP=Immunoprecipitation IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry ChIP=Chromatin IP
Reactivity Key: H=Human M=Mouse R=Rat B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IP IF-IC F ChIP	H M R (B)	Endogenous	75	Rabbit IgG

Protocols	4154: ChIP Agarose , ChIP Magnetic , Flow , Immunofluorescence , Immunoprecipitation , Western Blotting
Specificity / Sensitivity	EGR1 (44D5) Rabbit mAb detects endogenous levels of total EGR1 protein.
Source / Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of the sequence of human EGR1. Western Blotting Western analysis of cell extracts from serum starved PC12, untreated or NGF-treated (100 ng/ml for 2 hours), using EGR1 (44D5) Rabbit mAb. Flow Cytometry Flow cytometric analysis of PC-12 cells, untreated (blue) or NGF treated (green), using EGR1 (44D5) Rabbit mAb compared to a nonspecific negative control (red). IF-IC Confocal immunofluorescent analysis of dissociated PC12 cells either untreated (left) or NGF-treated for 2 hours (right), using EGR1 (44D5) Rabbit mAb (green) and MEK1/2 (L38C12) Mouse mAb (#4694) (red). Chromatin IP Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10⁶ THP-1 cells treated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (30ng/ml) overnight and either 10 μl of EGR1 (44D5) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP^® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ATAD2 promoter primers, SimpleChIP® Human EGR1 Promoter Primers #5549, human PCM1 promoter primers, and SimpleChIP^® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Background	EGR family members are transcriptional factors that contain three repetitive zinc finger DNA binding domains which bind to EGR response elements (ER) to regulate target gene expression (1). The expression of EGR family members is induced by growth factors, with EGR1 expression being induced by NGF (1,2). Increased EGR1 expression activates transcription of other signaling molecules, including CDK5 and tyrosine hydroxylase, and exerts long term effects on neural cell growth and differentiation (2,3). O'Donovan, K.J. et al. (1999) Trends Neurosci. 22, 167-173. Harada, T. et al. (2001) Nat. Cell Biol. 3, 453-459. Papanikolaou, N.A. and Sabban, E.L. (2000) J. Biol. Chem. 275, 26683-26689.
Application References	Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products	1015 EGR1 Blocking Peptide 4153 EGR1 (15F7) Rabbit mAb 2506 CDK5 Antibody 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody 7074 Anti-rabbit IgG, HRP-linked Antibody 7720 Prestained Protein Marker, Broad Range (Premixed Format) 7727 Biotinylated Protein Ladder Detection Pack 7003 20X LumiGLO^® Reagent and 20X Peroxide Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063. For Research Use Only. Not For Use In Diagnostic Procedures.