- 询价
- Cell Signaling Technology
- 4153
- USA
- 2026年05月28日
- W, IP, IHC-P, IF-IC, ChIP
- H,M,R,B
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- 文献和实验
- 技术资料
- 抗体英文名：
EGR1 (15F7) Rabbit mAb
- 抗原：
synthetic peptide corresponding to the amino terminus of the sequence of human EGR1
- 应用范围：
W, IP, IHC-P, IF-IC, ChIP
- 保质期：
详见说明书
- 适应物种：
H,M,R,B
- 库存：
大量
- 供应商：
CST
- 级别：
详见MSDS文件
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) ChIP=Chromatin IP
Reactivity Key: H=Human M=Mouse R=Rat B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-IC ChIP | H M R (B) | Endogenous | 75 | Rabbit IgG |
| Protocols | |
|---|---|
| Specificity / Sensitivity | EGR1 (15F7) Rabbit mAb detects endogenous levels of total EGR1 protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of the sequence of human EGR1. Western Blotting
Western analysis of extracts from serum starved PC12 cells, untreated or NGF-treated (100 ng/ml NGF for 2 hours), using EGR1 (15F7) Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded mouse brain using EGR1 (15F7) Rabbit mAb in the presence of control peptide (left) or EGR1 (15F7) Blocking Peptide #1015 (right). IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embeded human glioblastoma showing nuclear localization, using EGR1 (15F7) Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embeded human Non-Hodgkin's lymphoma, using EGR1 (15F7) Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded mouse brain, using EGR1 (15F7) Rabbit mAb. IF-IC
Confocal immunofluorescent analysis of dissociated PC12 cells either untreated (left) or NGF-treated for 2 hours (right) and labeled with EGR1 (44D5) Rabbit mAb (green) and MEK1/2 (L38C12) Mouse mAb #4694 (red). Chromatin IP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 THP-1 cells treated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (30ng/ml) overnight and either 10 μl of EGR1 (15F7) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ATAD2 promoter primers, SimpleChIP® Human EGR1 Promoter Primers #5549, human PCM1 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
| Background | EGR family members are transcriptional factors that contain three repetitive zinc finger DNA binding domains which bind to EGR response elements (ER) to regulate target gene expression (1). The expression of EGR family members is induced by growth factors, with EGR1 expression being induced by NGF (1,2). Increased EGR1 expression activates transcription of other signaling molecules, including CDK5 and tyrosine hydroxylase, and exerts long term effects on neural cell growth and differentiation (2,3). |
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063. For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Testing Rabbit Bleeds By Elisa
same amount of NaBH4 after 15''. Do a quick microfuge spin if there are too many bubbles. B. ELISA with Peptide conjugated to BSA Coat 10 ug/ml antigen (peptide conjugated to BSA) diluted in TBS (50 ul/well) ON at 4 deg C. Remove antigen
家兔（Rabbit,Oryctolagus curiculus）
家兔（Rabbit,Oryctolagus curiculus） 一、生物学特性和解剖生理特点 1．家兔属于哺乳纲、啮齿目、兔科、草食性哺乳动性。 2．家兔体小力弱、胆小怕惊、怕热、怕潮，喜欢安静、清洁、干燥、凉爽的环境，不能忍受污秽的条件。 3．是食草类（Herbivorous）单胃动物。饲养原则是以青粗食米为主，精饲料为辅。喜欢独居，白天活动少，都处于假眠或休息状态，夜间活动大，吃食多。有啃木、扒土的习惯
Immunofluorescence: Rabbit Anti-Murine RELMα
实验步骤 The following protocol used B6 mice that were infected with 200 embryonated Trichuris muris eggs and sacrificed 15 days post-infection. The tip of the cecum was removed, rinsed in 1X PBS pH 7.2, and fixed in 4% PFA
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