- 询价
- Cell Signaling Technology
- 2109
- USA
- 2025年08月04日
- W, IP, IHC-P, IHC-F, IF-F, IF-IC, F
- H,M,R,Hm,Mk,B,Pg,C
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Src (36D10) Rabbit mAb
- 抗原：
synthetic peptide corresponding to residues near the carboxy terminus of human Src
- 应用范围：
W, IP, IHC-P, IHC-F, IF-F, IF-IC, F
- 级别：
详见MSDS文件
- 适应物种：
H,M,R,Hm,Mk,B,Pg,C
- 库存：
大量
- 保质期：
详见说明书
- 供应商：
CST
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IF-F=Immunofluorescence (Frozen) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey C=Chicken B=Bovine Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IHC-F IF-F IF-IC F | H M R Hm Mk B Pg (C) | Endogenous | 60 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Src (36D10) Rabbit mAb detects endogenous levels of Src proteins. The antibody may cross-react with other Src family members, but does not cross-react with other non-family proteins. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Src. Western Blotting
Western blot analysis of extracts from various cell lines using Src (36D10) Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Src (36D10) Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded Non-Hodgkin's lymphoma using Src (36D10) Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinomausing Src (36D10) Rabbit mAb in the presence of control peptide (left) or Src Blocking Peptide #1235 (right). IHC-F (frozen)
Immunohistochemical analysis of frozen SKOV-3 xenograft using Src (36D10) Rabbit mAb. Flow Cytometry
Flow cytometric analysis of A-431 cells using Src (36D10) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). IF-IC
Confocal immunofluorescent analysis of MCF-7 cells labeled with Src (36D10) Rabbit mAb (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). IF-F
Confocal immunofluorescent analysis of rat colon labeled with Src (36D10) Rabbit mAb (red). Blue pseudocolor=DRAQ5® #4084 (fluorescent DNA dye). |
| Background | The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063. For Research Use Only. Not For Use In Diagnostic Procedures. |
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