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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Nuclease S7,from Staphylococcus aureus
- 保质期：
1年
- 保存条件：
运输wet ice
- 库存：
10⁺支
- 供应商：
上海睿安生物19121878899
- 规格：
15000 units/支
Roche货号10107921001核酸酶S7上海睿安生物19121878899默克Merck-Roche-Millipore-Sigma官方正式授权代理商
核酸酶S7
Micrococcal nuclease,from Staphylococcus aureus
别名:S7核酸酶
属性
biological source:Staphylococcus aureus
form:lyophilized
specific activity:~15000U/mg
packaging:pkg of 15000 U
manufacturer/tradename:Roche
optimum pH:7.5
shipped in:wet ice
Quality Level:100
说明
Application
- 从体外翻译系统中去除内源性RNA
- RNA测序实验
核酸酶S7已用于核糖体分析的核糖核酸酶选择过程研究。
Biochem/physiol Actions
核酸酶S7是一种核酸内切酶，主要切割单链底物。它还切割双链DNA或RNA。它切割RNA和DNA的5′磷酸二酯键，产生3′-单核苷酸和寡核苷酸。酶的活性取决于Ca2+ ，添加EDTA可使酶完全灭活。相比于DNA上的GC，核酸酶S7切割对富含AT的区域高度特异。
Preparation Note:未开封试剂储存于2-8°C；1mg/ml的稀释液则储存于-15 to -25°C。
Other Notes
一单位酶释放的足够量的酸溶性寡核苷酸可使A260增加1.0。
仅用于生命科学研究。不可用于诊断。
同行评审论文(部分:文献)
Organ-specific translation elongation rates measured by in vivo ribosome profiling.
Gerashchenko M V, et al.
bioRxiv, 279257 (2018)
High sequence specificity of micrococcal nuclease.
Dingwall C, et al.
Nucleic Acids Research, 9(12), 2659-2674 (1981)
Sequence specific cleavage of African green monkey ?-satellite DNA by micrococcal nuclease.
Horz W, et al.
Nucleic Acids Research, 11(13), 4275-4285 (1983)
Translation elongation rate varies among organs and decreases with age.
Maxim V Gerashchenko et al.
Nucleic acids research, 49(2), e9-e9 (2020-12-03)
There has been a surge of interest towards targeting protein synthesis to treat diseases and extend lifespan. Despite the progress, few options are available to assess translation in live animals, as their complexity limits the repertoire of experimental tools to
Ribonuclease selection for ribosome profiling.
Gerashchenko M V and Gladyshev V N
Nucleic Acids Research,45(2),e6-e6 (2016)
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文献和实验该产品被引用文献
Roche货号10107921001核酸酶S7上海睿安生物19121878899默克Merck-Roche-Millipore-Sigma官方正式授权代理商
核酸酶S7
Micrococcal nuclease,from Staphylococcus aureus
别名:S7核酸酶
属性
biological source:Staphylococcus aureus
form:lyophilized
specific activity:~15000U/mg
packaging:pkg of 15000 U
manufacturer/tradename:Roche
optimum pH:7.5
shipped in:wet ice
Quality Level:100
说明
Application
- 从体外翻译系统中去除内源性RNA
- RNA测序实验
核酸酶S7已用于核糖体分析的核糖核酸酶选择过程研究。
Biochem/physiol Actions
核酸酶S7是一种核酸内切酶，主要切割单链底物。它还切割双链DNA或RNA。它切割RNA和DNA的5′磷酸二酯键，产生3′-单核苷酸和寡核苷酸。酶的活性取决于Ca2+ ，添加EDTA可使酶完全灭活。相比于DNA上的GC，核酸酶S7切割对富含AT的区域高度特异。
Preparation Note:未开封试剂储存于2-8°C；1mg/ml的稀释液则储存于-15 to -25°C。
Other Notes
一单位酶释放的足够量的酸溶性寡核苷酸可使A260增加1.0。
仅用于生命科学研究。不可用于诊断。
同行评审论文(部分:文献)
Organ-specific translation elongation rates measured by in vivo ribosome profiling.
Gerashchenko M V, et al.
bioRxiv, 279257 (2018)
High sequence specificity of micrococcal nuclease.
Dingwall C, et al.
Nucleic Acids Research, 9(12), 2659-2674 (1981)
Sequence specific cleavage of African green monkey ?-satellite DNA by micrococcal nuclease.
Horz W, et al.
Nucleic Acids Research, 11(13), 4275-4285 (1983)
Translation elongation rate varies among organs and decreases with age.
Maxim V Gerashchenko et al.
Nucleic acids research, 49(2), e9-e9 (2020-12-03)
There has been a surge of interest towards targeting protein synthesis to treat diseases and extend lifespan. Despite the progress, few options are available to assess translation in live animals, as their complexity limits the repertoire of experimental tools to
Ribonuclease selection for ribosome profiling.
Gerashchenko M V and Gladyshev V N
Nucleic Acids Research,45(2),e6-e6 (2016)
文献支持
Roche货号10107921001核酸酶S7上海睿安生物19121878899默克Merck-Roche-Millipore-Sigma官方正式授权代理商
￥3341.62
