EZFluor™ UV Fluor Protein Gel

UV Shadowing

UV shadowing is a technique for visualizing nucleic acids separated on acrylamide/urea gels. The technique utilizes shortwave UV light (254 nm) and a fluor-coated TLC plate. We recommend UV shadowing as the method of choice for gel purification

Comassie Stain of Protein Gel

1.After electrophoresis of protein gel,transfer gel to round staining tray.Add ~200 ml protein gel stain. 2.Microwave for ~45 sec until the solution just starts to boil.Incubate at room temp with gentle shaking for 10-15 min.Heating allows the gel

Protocol Protein Gel Staining Using Coomassie Blue Protein Stain

  Materials Polyacrylamide pre-cast gel SimplyBlue™ Coomassie protein stain, 1x pre-mixed solution (Coomassie blue dye binds to proteins (arginine, the aromatic amino acids, and histidine) allowing protein bands to be visualized