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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
3年
- 英文名：
Hexokinase (420 U/ml) + G6P-DH (210 U/ml)
- 库存：
请咨询
- 供应商：
上海经科化学科技有限公司
- CAS号：
无
- 保存条件：
常温/冷藏
- 规格：
10mL
High purity Hexokinase (yeast) / Glucose-6-phosphate dehydrogenase (G6P-DH) (Leuconostoc mesenteroides) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
| Content: | 10 mL Hexokinase (420 U/mL) + G6P-DH (210 U/mL) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 1 year under recommended storage conditions |
| Enzyme Activity: | Kinase |
| EC Number: | Hexokinase: 2.7.1.1 G6P-DH: 1.1.1.49 |
| CAS Number: | Hexokinase: 9001-51-8 G6P-DH: 9001-40-5 |
| Synonyms: | Hexokinase: Hexokinase; ATP:D-hexose 6-phosphotransferase G6P-DH: glucose-6-phosphate dehydrogenase (NADP+); D-glucose-6-phosphate:NADP+ 1-oxidoreductase |
| Source: | Yeast |
| Concentration: | Hexokinase: ~ 194 U/mL G6P-DH: ~ 665 U/mL |
| Expression: | Hexokinase: Recombinant from yeast G6P-DH: Recombinant from Leuconostoc mesenteroides |
| Specificity: | Hexokinase: Catalyses the reaction: ATP + D-hexose = ADP + D-hexose 6-phosphate Phopshorylates D-glucose, D-mannose, D-fructose, sorbitol and D-glucosamine. Glucose-6-phosphate dehydrogenase: Catalyses the reaction: D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+ |
| Unit Definition: | Hexokinase: One Unit of hexokinase activity is defined as the amount of enzyme required to produce one µmole of NADH from NAD+ in the presence of D-glucose and glucose-6-phosphate dehydrogenase at pH 7.5 and 25oC. G6P-DH: One Unit of Glucose-6-phosphate dehydrogenase activity is the amount of enzyme required to convert one μmole of glucose-6-phosphate to 6-phosphogluconate per minute, in the presence of NADP+ at pH 7.8 and 25oC. |
| Temperature Optima: | 40oC |
| pH Optima: | 7.4 |
| Application examples: | Applications for the measurement of glucose and other hexoses in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries. |
如需己糖激酶/6-磷酸葡萄糖脱氢酶产品完整说明书，
请向上海经科客服索取！
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文献和实验1.Effect of non‐Saccharomyces yeast strains on 3‐isobutyl‐2‐methoxypyrazine concentration and aroma properties in Sauvignon Blanc wines during fermentation.
Li, J. C., Wilkinson, K. L., Ford, C. M. & Jiranek, V. (2022). Australian Journal of Grape and Wine Research, 28(4), 607-620.
2.Conservation of the glycogen metabolism pathway underlines a pivotal function of storage polysaccharides in Chlamydiae.
Colpaert, M., Kadouche, D., Ducatez, M., Pillonel, T., Kebbi-Beghdadi, C., Cenci, U., Huang, B., Chabi, M., Maes, E., Coddeville, B., Couderc, L., Touzet, H., Bray, F., Tirtiaux, C., Ball, S., Greub, G. & Colleoni, C. (2021). Communications Biology, 4(1), 1-16.
系可逆地催化醇脱氢形成醛（或酮）的酶，参与醇的发酵。 EC1． 1． 1． 1。 CH3 CH2 OH NAD CH3 CHO NADH H 。底物专一性较广，亦作用于其他的醇。除酵母外，也广泛存在于高等植物（特别在发芽时活性增强）、动物肝脏、细菌等生物界。已从酵母（ E． Negelein， H． J． Wulff1937）或肝脏中以结晶状态被提取出来。由酵母获得的结晶分子量约 15万，由 4个亚基组成，与 4个 NAD 及锌结合的 SH酶。肝脏
Respiration: Succinic Dehydrogenase
Materials Mitochondrial suspension from Exercise 8.4 Sodium dithionite 0.2 M Sorenson Phosphate Buffer, pH 7.5 1% (w/v) Bovine Serum Albumin 0.005 M
2O 酶法具有高效、反应专一的特点，还原反应可在常温下进行，不需要特殊环境。目前在市面上的快速血糖测定仪检测原理均属酶测定法，不同厂家所使用的酶种类不同。常用酶有葡萄糖脱氢酶Glucose dehydrogenase（GDH）、葡萄糖氧化酶Glucose oxidase （GO）、己糖激酶（常用于大生化仪）Hexokinase（HK）、6-磷酸葡萄糖脱氢酶Glucose-6-phosphate dehydrogenase等。 不同的酶具有不同的酶的特性。葡萄糖氧化酶对葡萄糖有特异性，但测量
