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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
> 2 years at 4oC.
- 英文名：
Galactose dehydrogenase / Galactose mutarotase
- 库存：
1
- 供应商：
上海经科化学科技有限公司
- 保存条件：
at 4oC.
- 规格：
1 mL
半乳糖脱氢酶-半乳糖变旋酶Galactose dehydrogenase - Galactose mutarotase介绍：
High purity Galactose dehydrogenase (Escherichia coli) / Galactose mutarotase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Can be employed for the rapid determination of D-galactose or L-arabinose. See Lactose/D-Galactose (Rapid) Assay Kit (K-LACGAR) for full details.
| Content: | 1 mL; galactose dehydrogenase (200 U/mL) / galactose mutarotase (4 mg/mL) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 1 year under recommended storage conditions |
| Enzyme Activity: | Dehydrogenase |
| EC Number: | Galactose dehydrogenase: 1.1.1.48 Galactose mutarotase: 5.1.3.3 |
| CAS Number: | Galactose dehydrogenase: 9028-54-0 Galactose mutarotase: 9031-76-9 |
| Synonyms: | Galactose dehydrogenase: D-galactose 1-dehydrogenase; D-galactose:NAD+ 1-oxidoreductase Galactose mutarotase: aldose 1-epimerase; aldose 1-epimerase |
| Source: | Escherichia coli |
| Expression: | Recombinant from Escherichia coli |
| Specificity: | Interconversion of the α- and β-anomeric forms of D-galactose is catalysed by β-galactose mutarotase (GMR) (1). (1) α-D-Galactose ↔ β-D-Galactose. The β-D-Galactose is oxidised by NAD+ to D-galactonic acid in the presence of β-galactose dehydrogenase (GALDH) at pH 7.5 (2). (2) β-D-Galactose + NAD+ → D-galactonic acid + NADH + H+ |
| Specific Activity: | Galactose dehydrogenase: ~ 200 U/mL at pH 8.6 and 25oC Galactose mutarotase: ~ 4 mg/mL |
| Unit Definition: | One Unit of galactose dehydrogenase is defined as the amount of enzyme required to produce one µmole of NADH from NAD+ per minute at pH 8.6, at 25oC. |
| Temperature Optima: | 25oC |
| pH Optima: | 8.6 |
| Application examples: | Used for the rapid measurement of D-galactose or L-arabinose in the L-Arabinose/D-Galactose Assay Kit (K-ARGA). Also used in the measurement of lactose via D-galactose in the Lactose/D-Galactose (Rapid) Assay Kit (K-LACGAR). |
半乳糖脱氢酶-半乳糖变旋酶Galactose dehydrogenase - Galactose mutarotase说明书(PDF)请向客服索取！
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文献和实验Charnock, S. C. & McCleary, B. V. (2006). Irish Patent No. S84280, UK Patent No. 1828407, German Patent No. 1828407, French Patent No. 1828407 and USA Patent No. US7, 785, 771/11/722,000.
2.Method for the Direct Determination of Available Carbohydrates in Low-Carbohydrate Products Using High-Performance Anion Exchange Chromatography.
Ellingson, D., Potts, B., Anderson, P., Burkhardt, G., Ellefson, W., Sullivan, D., Jacobs, W. & Ragan, R. (2010). Journal of AOAC International, 93(6), 1897-1904.
包含 xylA、xylB、xylR三种基因。xylA表达 D-木糖异构酶、xylB表达木酮糖激酶、xylR作为调节基因表达阻遏蛋白。xyl基因的变异使细胞不能以木糖作为碳源进行能量代谢。gal（Galactose）功能：gal基因表达半乳糖代谢所需的各种酶类及调节蛋白，包括： galE（17 min）表达尿苷二磷酸（UDG）半乳糖-4-差向异构酶、galK（17 min）表达半乳糖激酶、galP（64 min）表达半乳糖透性酶、galR（62 min）表达半乳糖操纵子的阻遏蛋白、galT（17
的半乳糖血症患者由于半乳糖-1-磷酸尿苷酰转移酶（galactose-1-phosphate uridy1 transferase,简称转移酶）缺乏（图4-22②），致使半乳糖-1-磷酸（Gal-1-P）及半乳糖积聚在血中，部分随尿排出。Gal-1-P在肝的积聚可引起肝功能损害，甚至肝硬化；在脑的积聚引起智力障碍；血中半乳糖升高可使葡萄糖释出减少，出现低血糖症。半乳糖在醛糖还原酶作用下产生半乳糖，能改变晶状的渗透压，使水分进入，影响晶状体代谢而致白内障。 患者都是隐性纯合子（gg），杂合
包含xylA、xylB、xylR三种基因。xylA表达D-木糖异构酶、xylB表达木酮糖激酶、xylR作为调节基因表达阻遏蛋白。xyl基因的变异使细胞不能以木糖作为碳源进行能量代谢。gal（Galactose）Map position：17 ~ 164min功能：gal基因表达半乳糖代谢所需的各种酶类及调节蛋白，包括：galE（17 min）表达尿苷二磷酸（UDG）半乳糖-4-差向异构酶、galK（17 min）表达半乳糖激酶、galP（64 min）表达半乳糖透性酶、galR（62 min）表达
