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- 2025年12月14日
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- 英文名：
endo-Polygalacturonanase
- 保质期：
1年
- 供应商：
上海经科化学科技有限公司
- 保存条件：
2-8℃
- 规格：
5,000 Units
High purity endo-Polygalacturonanase (Pectobacterium carotovorum) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
| Content: | 5,000 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 1 year under recommended storage conditions |
| Enzyme Activity: | endo-Polygalacturonase |
| EC Number: | 3.2.1.15 |
| CAZy Family: | GH28 |
| CAS Number: | 9032-75-1 |
| Synonyms: | polygalacturonase; (1→4)-alpha-D-galacturonan glycanohydrolase |
| Source: | Pectobacterium carotovorum |
| Molecular Weight: | 39,818 |
| Concentration: | Supplied at ~ 14,000 U/mL |
| Expression: | Recombinant from Pectobacterium carotovorum |
| Specificity: | Random hydrolysis of α-1,4-D-galactosiduronic linkages in pectate and polygalacturonans. |
| Specific Activity: | ~ 250 U/mg (40oC, pH 5.5 on polygalacturonic acid) |
| Unit Definition: | One Unit of endo-polygalacturonanase activity is defined as the amount of enzyme required to release one μmole of galacturonic acid from polygalacturonic acid (5 mg/mL) per min in sodium acetate buffer (100 mM), pH 5.5 at 40oC. |
| Temperature Optima: | 50oC |
| pH Optima: | 6 |
| Application examples: | Applications in carbohydrate research and in the food industry. |
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文献和实验1.Gelation behavior between Nicandra physalodes (Linn.) Gaertn. pectin and Ca2+ is governed by degree and pattern of methylesterification.
Zhang, H., Song, Z., Gong, T., Meng, Y., Wang, X., Zhang, S. & Guo, Y. (2024). Food Hydrocolloids, 149, 109640.
2.The model polysaccharide potato galactan is actually a mixture of different polysaccharides.
Feng, Z., Lin, Z., Tang, H., Geng, J., Hu, Y., Mayo, K. H., Tai, G. & Zhou, Y. (2023). Carbohydrate Polymers, 313, 120889.
3.MUR1‐mediated cell‐wall fucosylation is required for freezing tolerance in Arabidopsis thaliana.
Panter, P. E., Kent, O., Dale, M., Smith, S. J., Skipsey, M., Thorlby, G., Cummins, I., Ramsey, N., Begum, R. A., Sanhueza., Fry, S. C., Knight, M. R. & Fry, S. C. (2019). New Phytologist, 224(4), 1518-1531.
