pEnCMV-SP1(human)-HA-SV40-Neo

【求助】sp1siRNA转染细胞大家有做过的吗？

wuyf 我想看转录因子sp1对我的目的基因的表达是不是有影响，我用sp1siRNA转染细胞，发现与对照细胞相比，细胞内总RNA量减少。sp1siRNA转染细胞大家有做过的吗？ freecell 同样结果重复了几次么？做了几个剂量？每个剂量做了几个重复？ ffyy8888 :D因为转染了一些东西进去，或多或少会对细胞状态有影响的，你只要将对照做全，就可以呀，怕啥，多做几组对照

An Efficient Way of Studying ProteinProtein Interactions Involving HIF-, c-Myc, and Sp1

of mechanisms of cellular processes in biology and diseases. The hypoxia-inducible transcription factor HIF-1α possesses a non-transcriptional activity that competes with c-Myc for Sp1 binding, whereas its isoform HIF-2α lacks Sp1-binding activity

Qualitatively Monitoring Binding and Expression of the Transcription Factor Sp1 as a Useful Tool to Evaluate the Reliability of Primary Cultured Epith

in culture, they often lose their ability to proliferate, a process likely to be determined by the altered expression of nuclear-located transcription factors such as Sp1, whose expression has been documented to be required for cell adhesion, migration