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文献和实验Analysis of Testosterone in Serum Using Mass Spectrometry
For either gas chromatography mass spectrometry (GC-MS) or liquid chromatography tandem mass spectrometry (LC-MS-MS) methods, the first step in the analysis is to add a deuterium-labeled internal standard such as testosterone-16,16,17-d3
Human IL-2 Enzyme Immunoassay (EIA)
-2 EIA is an in vitro enzyme-linked immunosorbent assay for quantitatively detecting human IL-2 in biological fluids (e.g. serum, plasma) and in cell culture supernatants. The kit consists of a 96 well microtiter plate that has been precoated with anti-IL
such as sucrose. However, detergents such as sodium dodecyl sulfate and triton x-100 can interfere with the assay, as well as strongly alkaline solutions. *The following instructions are given for use with the BioRad model 2550 EIA reader
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