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文献和实验Analysis of Testosterone in Serum Using Mass Spectrometry
For either gas chromatography mass spectrometry (GC-MS) or liquid chromatography tandem mass spectrometry (LC-MS-MS) methods, the first step in the analysis is to add a deuterium-labeled internal standard such as testosterone-16,16,17-d3
, necessitating extensive autoradiographic exposure times (more than a week). Moreover, enhancement of signal would require enrichment of the telomerase fraction and additional time consumption (11 ).
in this way are already covalently fixed onto nylon membranes, and simply need a rinse in 5xSSPE before prehybridisation. Labelling PCR Products with Digoxigenin PCR products may be very conveniently labelled with digoxigenin-11-dUTP (Boehringer-Mannheim
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