- ¥6160
- BD
- 550026
- BD
- 2025年12月06日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
Mouse Immunoglobulin Isotyping Kit
- 规格：
100T
- 小鼠IG分型试剂盒 Catalog Number
- 550026
- Brand
- BD™ Cytometric Bead Array (CBA)
- Name
- Mouse Immunoglobulin Isotyping Kit
- Contents
- Reagents for capture of mouse IgG1, IgG2a, IgG2b, IgG3, IgA, IgM, and IgE; anti-kappa and anti-lambda detection reagents; standards; buffers; cytometer setup reagents
- Size
- 100 Tests
- Regulatory Status
- RUO
Description
The BD™ CBA system combines a conventional immunoassay with the sensitivity of fluorescence via flow cytometry. Each bead in a BD CBA provides a capture surface for a specific protein and is analogous to an individually coated well in an ELISA plate. The broad dynamic range of fluorescence detection, combined with multiple analyte detection, allows for small sample volume, fewer sample dilutions, and substantially less time overall to establish the value of an unknown versus a conventional ELISA approach. The Mouse Immunoglobulin Isotyping CBA Kit can be used to rapidly identify the heavy and light chain isotypes of a mouse monoclonal antibody in a single sample.
Components:
肿瘤研究，细胞因子，趋化因子，炎症因子，过敏毒素研究
BD生物科学一直致力于为科研工作者提供完整的流式解决方案。BDTMCBA(Cytometric Bead Array)技术是基于包被抗体的微球并利用流式细胞仪同时对多种蛋白质进行定量分析的创新性技术。每种微球具有特定的荧光强度，可以在一个试管中混合同 时进行检测。与传统的ELISA和Western Blot技术相比，该种方法需要的样本量小，检测速度更快。BDTMCBA试剂盒包括：固定待测指标预先组合的CBA kits、可配置检测方法、研究者可自由组合检测指标的CBA Flex kits。BDTMCBA技术可以应用于药物研发、免疫疾病（细胞因子）、肿瘤生物学等众多领域研究，它已经被全世界科研工作者广泛使用，相应发表的文献众多。
BD CBA技术的检测对象是分泌到液相样本中的可溶性蛋白，如细胞因子、趋化因子、炎症因子等等；目前固定好的试剂盒有Th1/Th2 CBA kit，Th1/Th2/Th17CBA kit，趋化因子CBA kit，炎症因子CBA kit及过敏毒素CBA kit等。自由组合的试剂可检测的种类 人的有61种，小鼠的27种，大鼠的6种。具体的因子如下：
| 人 | 小鼠 | 大鼠 | |
| IFN-γ,INF-α IL-1 α,IL-1β, IL-2,IL-3, IL-4 , IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-12p70, IL-13, IL-17A, IL-21, IL-23p40 TNF,TNFRⅠ,TNFRⅡ IP-10 I-TAC MIG MCP-1 MIP-1 α, MIP-1 β Eotaxin RANTES CD54(ICAM-1) CD62E,CD62P, CD62L CD106(VCAM-1) sCD40 sCD154(CD40 Ligand) |
sCD121a,sCD121b Soluble CD14 Basic FGF VEGF Angiogenin G-CSF,GM-CSF LIF LT-α OSM Granzyme A, Granzyme B Fas Ligand(FasL) Fractalkine IgA, IgG1, IgG2, IgG3, IgG4, IgM, Total IgG, |
IFN- γ IL-1 α ,IL-1β, IL-2,IL-3, IL-4 , IL-5, IL-6, IL-9, IL-10, IL-12, IL-12p70, IL-13, IL-17A, IL-21, IL-23p40, TNF MIG MCP-1 MIP-1 α, MIP-1 β RANTES KC CD62E(E-Selectin), CD62L(L-Selectin) G-CSF,GM-CSF |
IFN- γ IL-1 α, IL-4 , IL-6, IL-10 TNF |
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文献和实验Rapid Isotyping of Mouse Monoclonal Antibodies
for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance
实验步骤 1. Using the AbC™ Anti-Mouse Bead Kit 1) Completely resuspend the AbC™ capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2) Label
Reproducible purification of DNA and RNA in 96-well format. Genomic DNA and total RNA were purified from mouse tissues using the AllPrep DNA/RNA 96 Kit. A Purified DNA samples were analyzed on a 0.8% agarose gel. B
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