Mouse Th1/Th2 Cytokine Cytometric Bead Array (CBA) Kit
(RUO)描述
The BD™ CBA Mouse Th1/Th2 Cyokine Kit (Catalog No. 551287) can be used to measure Interleukin-2 (IL-2), Interleukin-4 (IL-4), Interleukin-5 (IL-5), Interferon-γ (IFN-γ), and Tumor Necrosis Factor (TNF) protein levels in a single sample. The kit performance has been optimized for analysis of physiologically relevant concentrations (pg/mL levels) of specific cytokine proteins in tissue culture supernatants and serum samples. The kit provides sufficient reagents for the quantitative analysis of 80 samples.
| Vial Label | Reagent | Quantity |
| A1 | Mouse IL-2 Capture Beads | 1 vial, 0.8 mL |
| A2 | Mouse IL-4 Capture Beads | 1 vial, 0.8 mL |
| A3 | Mouse IL-5 Capture Beads | 1 vial, 0.8 mL |
| A4 | Mouse IFN-γ Capture Beads | 1 vial, 0.8 mL |
| A5 | Mouse TNF Capture Beads | 1 vial, 0.8 mL |
| B | Mouse Th1/Th2 PE Detection Reagent | 1 vial, 4 mL |
| C | Mouse Th1/Th2 Cytokine Standards | 2 vials lyophilized |
| D | Cytometer Setup Beads | 1 vial, 1.5 mL |
| E | PE Positive Control Detector | 1 vial, 0.5 mL |
| E2 | FITC Positive Control Detector | 1 vial, 0.5 mL |
| F | Wash Buffer | 1 bottle, 130 mL |
| G | Assay Diluent | 1 bottle, 30 mL |
参考 (13)
Bishop JE, Davis KA. A flow cytometric immunoassay for β2-microglobulin in whole blood. J Immunol Methods. 1997; 210:79–87. (Biology).
Camilla C, Defoort JP, Delaage M, et al. A new flow cytometry-based multi-assay system 1, Application to cytokine immunoassays. Cytometry Suppl. 1998; 8:132. (Biology).
Carson R, Vignali D. Simultaneous quantitation of 15 cytokines using a multiplexed flow cytometric assay. J Immunol Methods. 1999; 227:41–52. (Biology).
Chen, R, L Lowe, et al. Simultaneous quantification of six human cytokines in a single sample using microparticle-based flow cytometric technology. Clin Chem. 1999; 9:1693-1694. (Biology).
Collins DP, Luebering BJ, Shaut DM. T-lymphocyte functionality assessed by analysis of cytokine receptor expression, intracellular cytokine expression, and femtomolar detection of cytokine secretion by quantitative flow cytometry. Cytometry. 1998; 33:249-255. (Biology).
Cook, EB, JL Stahl, et al. Simultaneous measurement of six cytokines in a single sample of human tears using microparticle-based flow cytometry: allergics vs non-allergics. J Immunol Methods. 2001; 254:109-118. (Biology).
Dotti, G, B Salvodo, et al. Adenovector-induced expression of human-CD40-ligand (hCD40L) by multiple myeloma cells: A model for immunotherapy. Exp Hematol. 2001; 29:952-961. (Biology).
Fulton RJ, McDade RL, Smith PL, Kienker LJ, Kettman JR Jr. Advanced multiplexed analysis with the FlowMetrix system. Clin Chem. 1997; 43:1749-1756. (Biology).
Kricka LJ. Diamandis EP, Christopoulos TK, ed. Immunoassay. Academic Press; 1996:389-404.
Lund-Johansen F, Davis K, Bishop J, de Waal Malefyt R. Flow cytometric analysis of immunoprecipitates: high-throughput analysis of protein phosphorylation and protein-protein interactions. Cytometry. 2000; 39(4):250-259. (Biology). 查看参考
McHugh TM. Flow microsphere immunoassay for the quantitative and simultaneous detection of multiple soluble analytes. Methods Cell Biol. 1994; 33:613-629. (Biology). 查看参考
Oliver KG, Kettman JR, Fulton RJ. Multiplexed analysis of human cytokines by use of the FlowMetrix system. Clin Chem. 1998; 44:2057-2060. (Biology).
Stall, A, Q Sun, et al. A single tube flow cytometric multibead assay for isotyping mouse monoclonal antibodies, Abstract LB77. Experimental Biology Meeting. 1998. (Biology).
