ezkine™Th1/Th17细胞因子试剂盒，全血

ezkine™Th1/Th17细胞因子试剂盒，全血
eZKine™ Th1/Th17 Whole Blood Intracellular Cytokine Kit

描述：这ezkine Th17细胞内细胞因子Th1／whole blood is designed to identify细胞因子试剂盒快速产生T细胞Th1和Th17 of the whole Lineages后外周血中的刺激的。刺激of whole blood in the presence of蛋白转运抑制剂鸡尾酒（CAT 00 4980，not included）是红细胞溶解and followed by固定在单步和随后的permeabilization。samples are then ready to染液with the Th1和Th17鸡尾酒/浓度的同种型匹配控制的鸡尾酒。

Th1细胞是CD4 + T细胞子集，重要的角色在宿主防御细菌和病毒对细胞内。天堂的承诺是controlled by the TH1转录因子T-bet和这些细胞是主要来源，stimulates of IFNγ，巨噬细胞，淋巴细胞，和pmns in the Destruction of细菌病原菌。IFNγalso helps Foster the development of细胞毒淋巴细胞（CTL和NK细胞）that are responsible for the细胞介导的细胞和肿瘤细胞对病毒的免疫反应。dysregulation of the Th1反应起着关键的作用，在许多炎症和自身免疫性疾病

Th17细胞play a key role in屏障防御病原菌对细胞外和真菌的即插即用such as a significant role in的自身免疫性疾病。they are defined by their表达的转录因子rorgt of the and the炎性细胞因子IL - 17A。through their激活和细胞因子的生产，促炎性信号，触发Th17促进中性粒细胞动员and the表达抗菌肽。一些塑性Th1和Th17 between the Lineages has been reported。此外，致病性Th17 subpopulation has been描述以及表达of both IL-17与IFNγ，as well as rorgt和T-bet。这些细胞是自身免疫性疾病和炎症implicated in several
使用文献References: Cosmi L, Maggi L, Santarlasci V, Liotta F, Annunziato F. T helper cells plasticity in inflammation. Cytometry A. 2013 Sep 5.

Shi G, Vistica BP, Nugent LF, Tan C, Wawrousek EF, Klinman DM, Gery I. Differential involvement of Th1 and Th17 in pathogenic autoimmune processes triggered by different TLR ligands. J Immunol. 2013 Jul 1;191(1):415-23.

Duhen R, Glatigny S, Arbelaez CA, Blair TC, Oukka M, Bettelli E. Cutting edge:the pathogenicity of IFN-gamma-producing Th17 cells is independent of T-bet. J Immunol. 2013 May 1;190(9):4478-82.

Lee YK, Turner H, Maynard CL, Oliver JR, Chen D, Elson CO, Weaver CT. Late developmental plasticity in the T helper 17 lineage. Immunity. 2009 Jan;30(1):92-107.

Bettelli E, Korn T, Oukka M, Kuchroo VK. Induction and effector functions of T(H)17 cells. Nature. 2008 Jun 19;453(7198):1051-7.

Reactivity	Human
Components	eZKine Th1/Th17 Cocktail (22-7780-71): 25 tests. Store at 4°C. This cocktail contains the following antibodies: Anti-Human CD3 (SK7) APC Anti-Human CD4 (SK3) PerCP-eFluor® 710 Anti-Human IL-17A (eBio64DEC17) PE Anti-Human IFN gamma (4S.B3) FITC eZKine Isotype Control Cocktail A (22-7781-71): 25 tests. Store at 4°C. This cocktail contains the following antibodies: Anti-Human CD3 (SK7) APC Anti-Human CD4 (SK3) PerCP-eFluor® 710 Mouse IgG1, K Isotype Control PE Mouse IgG1, K Isotype Control FITC eZKine Fix/Lyse Concentrate: 15 mL. Store at 4°C. Avoid agitation. This is a 4X stock solution that must be diluted prior to use with the eZKine Fix/Lyse Diluent. Dilute 1 part eZKine Fix/Lyse Concentrate with 3 parts eZKine Fix/Lyse Diluent. Caution: This solution contains formaldehyde, which is toxic and a suspected carcinogen. Contact with eyes, skin and mucous membranes should be avoided. Wear proper protective clothing and gloves. eZKine Fix/Lyse Diluent: 50 mL. Store at 4°C. The diluent is intended to be used in combination with the eZKine Fix/Lyse Concentrate. Permeabilization Buffer (10x): 20 mL. Dilute to 1X with deionized/distilled water and store at 4°C. Caution: Harmful if swallowed or irritant by contact. Wear proper protective clothing and gloves. Note: The Permeabilization Buffer (10X) has a natural tendency to precipitate, however, its function is not affected by this. To clarify, the solution can be filtered after dilution to 1X working solution.
Reported Applications	Intracellular Staining Followed by Flow Cytometric Analysis

Cat. No.	Name	Excite	Emit	Application	Reg.
00-4975	Cell Stimulation Cocktail (plus protein transport inhibitors) (500X)			FC, FA	GPR
11-7319	Anti-Human IFN gamma FITC	488 nm		FC	RUO
17-0036	Anti-Human CD3 APC	633 - 647 nm	660 nm	FC	RUO
01-1111	OneComp eBeads			FC	GPR
00-4980	Protein Transport Inhibitor Cocktail (500X)			FC, FA	GPR
12-7179	Anti-Human IL-17A PE	488 - 561 nm		FC	RUO
46-0047	Anti-Human CD4 PerCP-eFluor® 710	488 nm	710 nm	FC	RUO
8822-6856	eZKine™ 4-Color Compensation Control Kit			FC	RUO