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克隆是英文Clone一词的单译，意为无性繁殖系，即通过无性繁殖（如细胞丝分裂）可连续传代并形成的群体，常用于细胞水平的描述。克隆技术（Cloning）则指由众多的基因或细胞群体中通过无性繁殖和选择获得目的基因或细胞的技术操作。如基因克隆是指某种目的基因的分离过程，通常是将生物材料的遗传物质如DNA以酶切成片断，插入到载体中，通过无性繁殖（细菌或细胞的倍增）使其扩增，然后再以某种探针选择、钓取目的基 ...

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helvetica" size="2"Use of oligonucleotides in various research applications requires certain basic storage and handling techniques in order to ensure trouble-free experiments. Proper storage of your ...

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After an aliquot of the PCR mixture is analyzed on an agarose gel the remainder of the reaction is concentrated by ethanol precipitation resuspended in buffer and subjected to a simultaneous fi ...

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Ref. Seed and Sheen Gene 67 271-277 (1988) Normal reaction is Chloramphenicol + Acetyl-CoA -> Acetylchloramphenicol Assay uses butyryl-CoA (more hydrophobic) 14C-Cm+ Bu-CoA->Bu-14C-Cm Km (Cm) 1 ...

A. Culture Growth: 1.For BAC isolation for shotgun library construction: Pick a smear of colonies and inoculate 3 ml TB medium containing the appropriate antibiotic. Grow the culture for 8 hours 37 ...

Mutagenesis is a fundamentally important DNA technology which seeks to change the base sequence of DNA and test its effect on gene or DNA function. The mutagenesis can be conducted in vivo (in studies ...

1.grow up single colony in 1.5 ml LB/antibiotic ovn @37℃ . 2.pour into tube spin for 30 sec . 3.decant supernatant and resuspend pellet in 100 ml lysis solution . 4.add 200 ml alkaline SDS vortex well ...

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在分子生物学研究中，DNA 的序列分析是进一步研究和改造目的基因的基础。目前用于测序的技术主要有Sanger等（1977）发明的双脱氧链末端终止法和Maxam和 Gilbert（1977）发明的化学降解法。这二种方法在原理上差异很大，但都是根据核苷酸在某一固定的点开始，随机在某一个特定的碱基处终止，产生A，T，C，G四组不同长度的一系列核苷酸，然后在尿素变性的PAGE胶上电泳进行检测，从而获得DN ...