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alimama_pid="mm_10043573_137377_1822161"; alimama_titlecolor="FF9900"; alimama_descolor ="552c55"; alimama_bgcolor="f7f7f7"; alimama_bordercolor="f7f ...

基因工程：有目的的通过分子克隆技术，人为的操作改造基因，改变生物遗传性状的系列过程。 载体：能在连接酶的作用下和外源DNA片段连接并运送DNA分子进入受体细胞的DNA分子。 转化：指质粒DNA或以它为载体构建的重组DNA导入细菌的过程。 感染：以噬菌体、粘性质粒和真核细胞病毒为载体的重组DNA分子，在体外经过包装成具有感染能力的病毒或噬菌体颗粒，才能感染适当的细胞，并在细胞内扩增。 转导：指以噬菌 ...

alimama_pid="mm_10043573_137377_1822161"; alimama_titlecolor="FF9900"; alimama_descolor ="552c55"; alimama_bgcolor="f7f7f7"; alimama_bordercolor="f7f7f7"; alimama_linkcolor="552c55"; ...

alimama_pid="mm_10043573_137377_1822161"; alimama_titlecolor="FF9900"; alimama_descolor ="552c55"; alimama_bgcolor="f7f7f7"; alimama_bordercolor="f7f7f7"; alimama_linkcolor="552c55"; ...

alimama_pid="mm_10043573_137377_1822161"; alimama_titlecolor="FF9900"; alimama_descolor ="552c55"; alimama_bgcolor="f7f7f7"; alimama_bordercolor="f7f7f7"; alimama_linkcolor="552c55"; ...

This is a scaled up version of the Baron protocol which has been modified to achieve purity comparable to CsCl preps. I have not sorted out strain variations yet but HB101 works well. 1、Solutions Sol ...

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琼脂糖凝胶层析的技术数据 琼脂糖凝胶作为核酸检测主要也是理想材料，使用十分广泛，其理化性质一些参数如下表： （琼脂糖是琼脂内非离子型的组分，它在 0 ~ 4 ℃， pH 4~9 范围内是稳定的。） 名称、型号 ...

alimama_pid="mm_10043573_137377_1822161"; alimama_titlecolor="FF9900"; alimama_descolor ="552c55"; alimama_bgcolor="f7f7f7"; alimama_bordercolor="f7f7f7"; alimama_linkcolor="552c5 ...

10X TBE, per liter Sequencing dye ------------------- ---------------- 108g Tris base 80% formamide 55g boric acid 10mM NaOH 40ml 0.5M EDTA 1mM EDTA 0.1% xylene cyanol 10% APS 0.1% bromophenol blue ------------------- dissolve 0.1g ammonium persulfate in 10ml dH2O and store frozenGEL COMPOSITIONS per liter 6% 8% 20% ------------------------------------------------ acrylamide 57g 76g 190g bis acrylamide 3g 4g 10g urea 500g 500g 500g 10X TBE 100ml 100ml 100ml ddH2O 300ml 300ml 300ml

Phenol (removes protein) 1、add equal volume of Phenol (= tris-saturated Phenol-Chloroform-Isoamyethanol)2、vortex3、spin 2 minutes at 12000 rpm 4°C4、transfer supernatant to a fresh tube (avoi ...

1. Add an equal volume (equal to sample volume) of P/C to sample. 2. Mix (shake don't vortex). 3. Take aqueous (upper) layer. (If dirty sample repeat Ph/Chl step until interface is fairly clean). 4. A ...