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Post-transcriptional gene silencing (PTGS) which was initially considered a bizarre phenomenon limited to petunias and a few other plant species is now one of the hottest topics in molecular biology ( ...

Supplementary information to miRNAs cloned from mouse and human An online clearinghouse for miRNA gene name assignments (http://www.sanger.ac.uk/Software/Rfam/mirna/) is provided by the Rfam database ...

Note: Start with 20 mg of total RNA for each labeling reaction. All solutions that can be filtered should be filtered. Cy dyes are light sensitive and should ALWAYS be handled in dim light. RNA ...

Prepare in a sterile tube: template RNA: total RNA 0.1-5µg or poly(A)+ mRNA 10ng-0.5µg or specific RNA 0.01pg-0.5µg primer: oligo(dT)18 0.5µg or ...

The Easiest Route to Guaranteed Silencing Increasing numbers of researchers are using small interfering RNAs (siRNAs) to reduce the expression of specific mammalian genes. Applications of siRNA induce ...

The following protocol is for MEGAscript II Kit (Ambion). 1. PCR amplify the DNA template. The 5'-end of the template should contain the minimum promoter sequenences of T7 or Sp6 or T3. A 5-primer wi ...

in vitro Transcription Reaction 1 µl 10X Transcription Buffer (Ambion) 1 µl 10X NTPs (4 mM ATP CTP 1 mM GTP UTP) 2 µl 10 mM GpppG cap (Pharmacia) 2 µl a-UTP (NEN) 800 Ci/mmo ...

The siRNA user guide (revised May 6 2004) Selection of siRNA duplexes from the target mRNA sequence Using Drosophila melanogaster lysates (Tuschl et al. 1999) we have systematically ...

Transcription of cRNA probe Reagents/Solutions DNA (PCR or plasmid) with bacteriophage RNA polymerase promoter in suitable orientation (see note 1 ) ~1µg/µl in TE buffer 10mM MgCl2 5x Tr ...

The ribonuclease protection assay (RPA) is a highly sensitive and specific method for the detection of mRNA species. The assay was made possible by the discovery and characterization of DNA-dependant ...

Secondary structure of prion mRNA Luck R; Steger G; Riesner D Institut fur Physikalische Biologie Heinrich-Heine-Universitat Dusseldorf FRG. J Mol Biol 258: 813-26 (1996) An algorithm for prediction ...

All solutions used in RNA preparation should be treated with DEPC as follows: add DEPC as follows: add DEPC to 0.1% and vigorously stir for one hour to O/N followed by autoclaving for 30 minutes ...

RNAi相关文章（for free） Prospects of RNA interference therapy for cancer S IPai Y-YLin BMacaes AMeneshian C-FHung and T-CWu Gene Ther 13: 464-477; ...

(1) Core out a single well separated plaque from the agar plate using a 200µl pipette tip and place it into 500µl of SM buffer in a 1.5ml microtube: SM buffer 5.8g of NaCl 2.0g of M ...

Using siRNA for gene silencing is a rapidly evolving tool in molecular biology. There are several methods for preparing siRNA such as chemical synthesis in vitro transcription siRNA expression vectors ...

We use the Promega Ribomax Large Scale RNA Production System T7 (Cat. No.: P1300) to produce dsRNAs and the RNAi Exp.html" target=_blank> Jack Dixon protocol ( RNAi _Dixon.html" target=_b ...

In the past poly(A)+ RNA has not been detected in prokaryotes. In the early 80s a new method of isolating RNA from bacteria was developed involving lysis by protease K in the presence of SDS and ...

Background Information of RNA i RNA interference (RNA i) is a biological process in which the introduction of double-stranded RNA (dsRNA ) into a cell results in targeted post-tranional gene silencing ...

RNA for S1 or PE analysis must be free of chromosomal DNA. This can be accomplished by extraction of the RNA with hot phenol but phenol can especially if its pH is acidic cause the specific loss ...

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