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(adapted from Bruce A. Roe Department of Chemistry and Biochemistry The University of Oklahoma Norman Oklahoma 73019 broe@ou.edu) Typically 2.5 - 3 volumes of an ethanol/acetate solution is added to t ...

Hello I have been trying over the last few months to introduce a single point mutation in a looped portion of an RNA using the dut-ung method of mutagenesis. So far I have had little success.&nb ...

A practical method for the preparation of total DNA from filamentous fungi M.I. Borges M.O. Azevedo R. Bonatelli Jr. M.S.S. Felipe and S. Astolfi-Filho Departamento de Biologia Celular Universidade de ...

Electroelution of agarose fragments Electroelution buffer 1 M Tris pH 7.5 12.0 mls 0.5 M EDTA 0.24 mls 1 M NaCl 3.0 mls qs to 600 mls dH2O Acetate cushion 3 M NaAcetate pH 4.8 480 μl 0.1 % Bromphe ...

I have tried to purify my PCR products by using 3 different approaches: 1) QIAQuick PCR purification kit: but when I run the gel to see its efficacy there was simply nothing!! it cleaned everything in ...

Kitto Lab The University of Texas at Austin http://research.cm.utexas.edu/bkitto/Kittolabpage/Protocols/Microbiology/electroporation.html This procedure prepares glycerol stock cultures of bacteria f ...

Kitto Lab The University of Texas at Austin http://research.cm.utexas.edu/bkitto/Kittolabpage/Protocols/Microbiology/ethanolPpt.html This procedure allows the concentration of DNA samples from dilute ...

Mitochondrial DNA Isolation from Somatic Embryogenic Cell Cultures of Larix (Excerpt from Thesis). Mitochondrial DNA is isolated by a modification of the methods described by Wilson and Chourey (1984) ...

Preparation of Sonicated Salmon Sperm DNA Procedure: 1) Use Pharmacia #27-4564-01. With clean flamed scissors and forceps weigh 0.25 g/50 ml conical and add 50 ml/conical of 0.02 M Tris pH 7.6. Allo ...

The Preuss LabThe Division of Biological SciencesThe University of Chicago http://preuss.bsd.uchicago.edu/protocols/topo.html ...

Contributed by Dr. A. Gratchev Single Nucleotide Primer Extension is a powerful method which can be used for the precise analysis of methylation in a certain position. The procedure is shown on the fi ...

Hi all! We are trying to extract genomic DNA from the leaves of an Ericacea (we suppose it is rich in tanins and phenolics) and we can't do it! We have used two different kits which work OK for other ...

Source: Protocol Online Abstract: Modifying DNA using sodium bisulfite to convert unmethylated cytosines to uracils and subsequently detect methylated cytosines using methylation specific PCR (MSP) te ...

1. Transfer plugs of mycelial margin to 0.1 P plates. Allow to grow to fill plate (about one week at 21°). 2. Macerate two plates of mycelia in a blender with 50 ml of .002 P liquid medium. Inocul ...

- make a 5µm section to do an evaluation of the % tumour cells - make 50X50µm sections for the DNA isolation - put sections in a falcon tube containing 10 ml of 1XSE - add 100µg/ml p ...

1.Vectorpedia： 输入质粒骨架，直接查询，非常方便; http://www.addgene.org/pgvec1?f=v&cmd=listvecinfo 2. google 检索式子：质粒名 ＋ map 质粒名 ＋sequence ＋filetype:txt or filetype:pdf 或者：进入google，点击"图片搜索"，直接查找图谱。 3.go ...

LiAc/SS-DNA/PEG TRANSFORMATION TRAFO Efficiency: up to 22 million transformanst/ µg of plasmid DNA HIGH EFFICIENCY TRANSFORMATION Please cite: Agatep R. R.D. Kirkpatrick D.L. Parchaliuk R ...

Preparation of plasmid DNA : a modified mini alkaline-lysis/PEG precipitation procedure. ABI; 1995 Materials GTE buffer (50mM glucose 25mM Tris-HCl (pH8.0) 10mM EDTA (pH 8.0)) (200µl/tube) 0.2N ...

Abstract: Single strand conformational polymorphism (SSCP)is the most widely used PCR-based methods for point mutation detection. The abnormal band found by SSCP analysis is normally verified ...