• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        DNA extraction from urine-Molecular Biology

        互联网

        928

        Does anyone have expereince of extracting DNA from urine? I am having problems with this. I have previously extracted the DNA but then it degraded very quickly and since then I havent even been able to extract DNA. I have been using a Qiagen extraction kit that is suitable for urine. without any success. Can anyone help?

        -molmicro-

        --------------------------------------------------------------------------------

        Urine must always be charged with 15 ug/ml proteinaseK to prevent destruction of DNA by lurking nucleases. It can be removed with phenol later.

        -phdconsult-

        --------------------------------------------------------------------------------

        hi dear

        i have also tried to extrct DNA from using the qiagen midi column to some extent i got sucssess but my product degraded after one week

        on the very fist hour after extrction i cheked DNA on gel it showed very light band t after one week i repeated the same electrophoresis bu no band observed althoug the DNA was stored in proper condition

        can u please let me kno hw u r approaching the sample

        then may be i can help u

        al the best

        -nitin-

        --------------------------------------------------------------------------------

        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序