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Amberg Lab Upstate Medical University http://www.upstate.edu/biochem/amberg/protocols/transformation.html 1.Grow cells to 1X10E8 or OD600 of 1.2-1.3. 2.Spin cells at 5000rpm for 5 min and wash pelle ...

Hi....I need a prot℃ol for extracting DNA from hair/roots...(specifically horse)...and any additional information such as ?storage pr℃edure/timing/etc....thanks.... -tree- --------------------------- ...

Submission Details: *Dr. Simon DawsoundefinedDepartment of BiochemistrundefinedUniversity of NottinghaundefinedU.K.*17:0undefined22/1/96. Database: Method/Protocol-->Spin column purification can be used to change buffers with ...

In the hood: 96 well dish with bacteria titertech microtubes glass pipette Remove colonies from each well using the titertech and place them into the cover Pipette up and down to thoroughly mix the co ...

Rapid elution of DNA from agarose gels James Movius Hahn Lab Last modified Sun Nov 1 1998 A method of quickly purifying agarose gel DNA fragments for use in subsequent reactions such as further restr ...

Author: Suzanne Gerttula Date: March 141994 1.Digest between 1 and 10 μg Genomic DNA. I digest at 37 C for about six hours over the course of a day. Spin down briefly heat to 65℃ for 10 min ...

Abstract: It is very difficult to isolate Rhizobium DNA due to the gum production by the organism. Hence we have designed a protocol for efficient isolation of DNA from the organism for further gene a ...

Hi I have a 9kb fragment already cloned into bluescript and I am trying to insert an extra 3.5kb. I've been trying for some time now with no success - when I do get colonies very few have any of the n ...

双元载体的农杆菌转化 1.1农杆菌感受态细胞的制备 1.1.1. 取-70℃保存的EHA105于含50μg/ml链霉素平板划线，28℃培养。 1.1.2. 挑取单菌落接种于5ml YM液体培养基中，220rpm 28℃振荡培养12-16 hr。 1.1.3. 取2ml菌液转接于100ml YM液体培养基中，28℃220rpm振荡培养至OD600=0.5。 1.1.4. 转入无菌离心管，500 ...

试验准备： 1、LB液体培养基(Luria-Bertani) ：称取蛋白胨(Tryptone)10 g，酵母提取物(Yeast extract) 5 g，NaCl 10 g，溶于800ml去离子水中，用NaOH调pH至7.5 加去离子水至总体积1升高压下蒸气灭菌20分钟。 2、LB固体培养基：液体培养基中每升加12g琼脂粉高压灭菌。 3、氨苄青霉素(Ampicillin Amp)母液：配成 50 ...

In situ immunodetection of 5-methylcytosine (5-mC) on squashed cells using anti-methylcytosine Cross-references are to Schwarzacher & Heslop-Harrison 2000. Practical in situ Hybridization. Bios Ox ...

Hi everyone i'm looking for a good southern blot protocol with all the secrets of this technique. I never did it but I know is not too difficult. I'd like a protocol that show me the "way to do& ...

Higuys : I have a problem about mutagenesis. I want to mutate 2 base pairs in plasmidand use stratagenne mutagenesis protocol.Design 2 oligos as primers.After I run PCR I only see one band less than ...

This is a four day procedure so it's best to start on Monday or Tuesday. CASE SELECTION: H&E stained thin sections are first reviewed by a pathologist and areas of interest are outlined. Tissue bl ...

Inoculate 1 ml of L-broth or other rich media with cells of the strain from which genomic DNA is to be isolated. Grow this culture at 37℃ overnight on a roller. Transfer this overnight culture to a 1. ...

(adapted from Bruce A. Roe Department of Chemistry and Biochemistry The University of Oklahoma Norman Oklahoma 73019 broe@ou.edu) Restriction enzyme digestions are performed by incubating double-stran ...

1.Take the 384 well viper plate from the thermocycler. Remove the Silicone Sealing Mat (Axygen Scientific: AM-384-PCR-RD) and place face down on a paper towel and pat to absorb any liquid that may be ...

GEL PURIFICATION OF OLIGONUCLEOTIDES (Adapted from the method of Mark Fortini) 1. Quantitate crude oligo solution via UV spectrophotometry. Assume 1.0 A260 unit is equal to 33μl/ml. Dry down 100&mu ...

随机扩增多态DNA (Random Amplified Polymorphic DNA ，简称RAPD)是J.Williams和J.Welsh两个研究小组于1990年同时提出的一种随机引物扩增，寻找多态DNA 片段的遗传标记技术，它是建立在PCR技术基础上，以随机的寡聚脱氧核苷酸作为PCR反应引物，对基因DNA 进行扩增而显示DNA 图谱和对物种进行亲缘关系、系统发育分子水平的鉴别，以及分子生物学 ...

分子诊断技术在检验医学的基础和临床研究中显示出强大的优势，例如，在乙型肝炎的诊断和治疗中，应用DNA定量技术为乙型肝炎的治疗和预后监测提供了重要依据，但近近不无症状乙型肝炎表面抗原携带者及乙型肝炎表面抗阴性者的人群中检出了前C终止密码子变异（G1896A）。研究表明，突变将导致乙型肝炎病毒继续复制，因此这一信息对于临床诊疗十分重要。又如庆大霉素等氨基糖苷类抗生素的毒副作用之一是诱发耳聋，且已证明线 ...