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Bradford Protein Assay- a microassay for determining protein content in a 96-well microtiter plate format Laboratory of P.J.HansenDept.of Animal SciencesUniversity of Florida http://www.animal.ufl.edu ...

This is a protocol from Engelke et.al.(1990)which was modified by Dr.Baron.The enzyme has been used for RT-PCRgenotyping and cloning. Solutions 1000X IPTG 0.5M IPTG 1.19g IPTG up to 10 ml wih sterile ...

常用核酸和氨基酸代码 Amino Acids Table Here is a list of the standard one-letter amino acid codes and their three-letter equivalents. The synonymous codons and their depiction in the IUB codes are shown. You sh ...

Hahn Lab (adapted from J.LeatherwoodPtashne lab) last modified MonAug 272001 Cell Growth 3 liters of cells are grown in YPD (3% glucose)to A600 of 3-5.Antifoam A (two drops from a P200 pipetman)is ad ...

Procedure A: Preparation of the cell lysate 1.Rinse a 60 mm culture dish of confluent cells with 10 mM Tris-HClPH 7.40.15 M NaCl1 mM MnCl2 and 0.2 mM PMSF. 2.Lyse the cells with 0.5ml cold buffer (1 ...

Before trying this methodtry rapid protein prep which involves boiling yeast in SDS PAGE buffer 1.Grow 100 ml yeast to A600 ~1.0.If the yeast are overgrownuse proportionally less cells for the prep. 2 ...

Use Promega's nuclease-treated -Met (L4210)or -Cys (L4240).The lysate is the samejust the amino acid mixes differ.The Promega protocols work fine as followsalthough it is often best to scale them down ...

Purpse The prtcl describes hw t prepare human tnsil lysate fr use in purificatin f ICAM-1LFA-1and PNAd. Materials Safety Equipments Lab Cat Latex Glves Face Mask Bench Paper Fresh human tnsil tissue ...

Contributor: Suprya Jayadev Date: September 211993 Resin preparation: 1)Measure out appropriate volume of resin into a 50 ml conical tube. --> NOTE: 1 ml of DEAE-Sephacel should bind approximately ...

Hattoti's protocol adapted to cell culture : Hattori MTugores AVeloz LKarin MBrenner D (1990)A simplified method for the preparation of transcrip-tionally active liver nuclear extracts.DNA Cell Biol.V ...

目前常用的离子交换纤维素列于下表 DEAE － 纤维素 形状 长度 （微米） ...

This method was successful in our lab using prostate tissue and for our specific objectives.Investigators must be aware that they will need to tailor the following protocol for their own research obje ...

Solutions Elution Buffer 50 mM Tris 7.5 5 ml 1M Tris pH 7.5 0.1% SDS 0.1 g SDS 0.1 mg/ml BSA 1.0 mg BSA 0.25 mM EDTA 50 ml 0.5M EDTA 2.5% glycerol 2.5 ml glycerol up to 100 ml with Q For every 10 ml a ...

Glutathione- a microassay for determining glutathione content in cells 检测细胞谷胱苷肽含量 Laboratory of P.J.HansenDept.of Animal SciencesUniversity of Florida http://www.animal.ufl.edu/hansen/protocols/gsh.ht ...

TCA-DOC For precipitation of very low protein concentration 1)To one volume of protein solutionadd 1/100 vol.of 2% DOC (Na deoxycholatedetergent). 2)Vortex and let sit for 30min at 4ºC. 3)Add 1 ...

Preparation of Affinity Column SEK 5/3/95 1.Add 222.2μl 1M MOPSpH 7.5 to 2ml of 10mg of CREBtide (0.1M MOPS pH 7.5 final concentration). 2.Read OD205OD280 (using 0.1M MOPS buffer as blank) 3.Affi ...

薄层色谱法，系将适宜的固定相涂布于玻璃板、塑料或铝基片上，成一均匀薄层。待点样、展开后，与适宜的对照物按同法所得的色谱图作对比，用以进行药品的鉴别、杂质检查或含量测定的方法。 1.仪器与材料 (1)玻板 除另有规定外，用5cm×20cm，10cm×20cm或20cm×20cm的规格要求光滑、平整，洗净后不附水珠，晾干。 (2)固定相或载体 最常用的有硅胶G、硅胶 ...

分析者对待测离子应有一些一般信息，首先应了解待测化合物的分子结构和性质以及样品的基体情况，如无机还是有机离子，离子的电荷数，是酸还是碱，亲水还是疏水，是否为表面活性化合物等。待测离子的疏水性和水合能是决定选用何种分离方式的主要因素。水合能高和疏水性弱的离子，如Cl-或K+，最好用HPIC分离。水合能低和疏水性强的离子，如高氯酸（ClO4-）或四丁基铵，最好用亲水性强的离子交换分离柱或MPIC分离。 ...

蛋白质浓缩技术是免疫学中常用的手段，现介绍几种常用的浓缩技术。 （一）透析袋浓缩法 利用透析袋浓缩蛋白质溶液是应用最广的一种。将要浓缩的蛋白溶液放入透析袋（无透析袋可用玻璃纸代替），结扎，把高分子（6 000－12 000）聚合物如聚乙二醇（碳蜡）、聚乙烯吡咯、烷酮等或蔗糖撒在透析袋外即可。也可将吸水剂配成30%－40%浓度的溶液，将装有蛋白液的透析袋放入即可。吸水剂用过后，可放入温箱中烘干或自然 ...

传统方法难以对蛋白质进行分离且保持其活性，但采用连在磁珠上的单克隆抗体进行免疫沉淀富集，SDS-PAGE电泳法检测则可达想要结果。用磁珠分离细胞溶菌液中的蛋白 质，几乎不需要预先处理，与其他方法相比，非特异性结合也较少。 要从全血，培养上清液，血清，腹水中分离蛋白质，用于制备、分析，运用磁珠作为固相载体进行免疫测定的优点在于快速的结合动力学和简单的分离，洗涤过程。在蛋白质纯化中，为了得到高结合容量 ...