全部

简介： 这是一本很实用的细胞培养方面的书！ 【书籍名称】：《细胞培养》 【书籍类别】：实验技术 【书籍格式】：PDF 【书籍来源】：主编司徒振强 【书籍大小】：6.4M 【书籍描述】：内容简介 第一章 细胞培养的一般知识 第二章 培养室的设备、设置和准备工作 第三章 培养用液及培养基 第四章 基本技术 第五章 正常组织细胞的培养 第六章 肿瘤细胞的培养 第七章 培养细胞生长状况的观察 第八章 细 ...

1.Crosslink protein DNA complexes in vivo Grow cells in suspension and collect 5 x108 cells by low speed centrifugation.Resuspend cells in 50ml media.In fume hoodadd 1.4ml 37% fomaldehyde solution to ...

1.Two rounds of DNA synthesis to incorporate fixed sequences Prepare the following: Reaction tube containing the following: 7ml DNA to be amplified and labelled 2ml 5X T7 Sequenase Buffer 1ml 50mM Pim ...

This method uses carboxyfluorescein succinimidyl ester (CFSE)rather than fluorescein isothiocyanateresulting in more reliable labeling.Succinimidyl esters are excellent reagents for amine modification ...

Day 0 and 1 Materials 1.0.5 mM ATP0.2 mM CaCl22 mM Tris-HClpH 8.0 at 4℃250 ml for day 0. 2.100 mM KCl100 mM boric acidpH 8.4 at 4℃10 ml. 3.0.5 mM ATP2 mM MgCl2100 mM KCl2 mM DTT2 mM PIPESpH 7.01000 ml ...

Day 0 and 1 Materials 1.0.5 mM ATP0.2 mM CaCl21 mM PIPESpH 6.8 at 4℃250 ml for day 0. 2.0.5 mM ATP0.2 mM CaCl270 mM KCl150 mM PIPESpH 7.01 ml. 3.16 mM ATP0.2 mM CaCl25 mM DTT5 mM lysine10 mM glutamic ...

Day 0 and 1 Materials 1.0.5 mM ATP0.2 mM CaCl22 mM Tris-HClpH 8.0 at 4℃250 ml for day 0 and 1000 ml for day 1. 2.100 mM KCl100 mM boric acidpH 8.4 at 4℃10 ml. 3.IATR (tetramethylrhodamine iodoacetamid ...

1、侵袭实验与转染细胞问题。 问：我要做侵袭实验，但我做的是瞬时转染的贴壁细胞，我想请教各位，瞬时转染后多长时间，可以消化细胞进行transwell小室的细胞接种？ 答：最好是24小时，我们科有两个都用二十四小时，但也有文献报道说用48小时。如果是稳定转染，则需要在对数生长期接种细胞。 2、原代成骨细胞培养不贴壁问题。 问：我用新配置的0.25%胰蛋白酶消化骨片30分，然后用胶原酶消化90分，培养 ...

Day 0 and 1 Materials 1.0.5 mM ATP0.2 mM CaCl22 mM Tris-HClpH 8.0 at 4℃250 ml for day 0. 2.0.5 mM ATP2 mM MgCl2100 mM boric acidpH 8.3 at 4℃10 ml. 3.N-(1-pyrene)iodoacetamide (Molecular probes P-29m.w ...

Day 1-2 Materials 1.0.5 M KCl10 mM HEPESpH 7.54℃250 ml. 2.0.5 M KCl50 mM HEPESpH 8.04℃250 ml. 3.Bio-Beads SM-2 in a 0.7x15 column. 4.20 mM KCl20 mM PIPESpH 7.04℃250 ml. 5.IATR (tetramethylrhodamine io ...

Day 1 Materials 1.50 mM KCl0.2 mM EGTA2 mM ATP2 mM MgCl210 mM HEPESpH 7.0.Need 500 ml. Procedure 1.Thaw appropriate volume of frozen gizzard myosin to obtain 10-20 mg. 2.Centrifuge in a SS34 rotor at ...

Materials 1.Small vial and stir bar. 2.Bio-Beads SM-2 in 0.7x15 cm column. 3.2 mM Tris-HClpH 8.54℃250 ml. 4.Centricon-30 concentrator. 5.5 mM Tris-acetate0.1 mM DTTpH 6.954℃250 ml. 6.200 mM K-boratepH ...

Materials 1.200 mM K-boratepH 9.010 ml. 2.100 mM lysine100 mM K-boratepH 9.01 ml aliquots stored at -20℃. 3.2 mM Tris-HClpH 8.54℃250 ml. 4.100 mM DTT stock. 5.Centricon-30 concentrator. 6.5 mM Tris-ac ...

Materials 1.Drop frozen 2x cycled tubulin in a polymerization-promoting buffer.Stored at -80℃ 2.GTP100 mM stocktitrate to pH ~7.Need 2 ml. 3.Waterbathset at 37℃ 4.SS34 rotor5 ml and 15 ml tubes and ad ...

Materials 1.2 mM PIPESpH 7.0500 ml. 2.Gc-globulin (Calbiochem 345802)1 mg. 3.CFSE (Carboxyfluorescein succinimidyl ester; Molecular Probes). 4.Lysine100 mM in buffer 110 ml. 5.G25-150 desalting column ...

Considerations for use The Bradford assay is very fast and uses about the same amount of protein as the Lowry assay.It is fairly accurate and samples that are out of range can be retested within minut ...

Considerations for use The principle of the biuret assay is similar to that of the Lowryhowever it involves a single incubation of 20 min.There are very few interfering agents (ammonium salts being on ...

Considerations for use The bicinchoninic acid (BCA)assay is available in kit form from Pierce (RockfordIll.).This procedure is very applicable to microtiter plate methods.The BCA is used for the same ...

How long should cells be labeled? The ideal length of time to label cells depends on the protein of interest and the label that you are using.If you want to label an unstable protein with 35S-methioni ...

Recipes for the buffers are at the end of this protocol. 1.Label your protein with 32Pi.Then subject the protein to SDS polyacrylamide gel electrophoresis and transfer your gel-fractionated protein to ...