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目的： 学习水平式琼脂糖凝胶电泳，检测质粒DNA的构型、分子量的大小。 原理： 琼脂糖是从海藻中提取出来的一种线状高聚物，可作为电泳支持物，适用于分离大小范围在0.2-50kb的DNA片段。DNA分子的迁移率与分子量的对数值成反比关系。观察其迁移距离，与标准DNA片段进行对照，就可获知该样品分子量大小。在质粒抽提过程中，由于各种因素的影响，使质粒DNA呈现超螺旋的共价闭合环状、开环状分子、线状分子 ...

实验目的 学习水平式琼脂糖凝胶电泳检测DNA的纯度、构型、含量和分子量大小。 实验原理 闭合环状质粒、线性质粒和开环质粒DNA由于构形不同，在加溴化乙锭的琼脂糖凝胶电泳上呈现不同的迁移率，因而在紫外灯下观察，能区别闭合环状质粒DNA（cccDNA）、线性质粒DNA（L-DNA）和开环质粒DNA（ocDNA）。 实验材料和试剂 （一）实验样品 质粒pUC118 （二）试剂 1．l DN ...

名称：公用动物实验室标准操作及管理规程(SOP) 关键词：实验动物 实验室 操作管理规则 目的：严格控制动物实验环境不受病源微生物的危害；保护动物的福利；维持环境的生物洁净度；确保动物使用与实验环境的一致性。 背景知识（选填项目）： 原理（选填项目）： 主体内容： （1）实验人员和本室工作人员进入开放系统动物室，应着工作服，并保持动物饲养室和公用动物实验室的整洁、安静。无关人员未经许可，不得进入。 ...

TITLE: Document Controls SCOPE: All Divisional Personnel RESPONSIBILITY: Program Administrative Personnel PURPOSE: To Outline the Proper Procedures to Control all Policy Procedureand Recording Documen ...

一、原理 细菌体中含有大量蛋白质，具有不同的电荷和分子量。强阴离子去污剂SDS与某一还原剂并用，通过加热使蛋白质解离，大量的SDS结合蛋白质，使其带相同密度的负电荷，在聚丙烯酰胺凝胶电泳（PAGE）上，不同蛋白质的迁移率仅取决于分子量。采用考马斯亮兰快速染色，可及时观察电泳分离效果。因而根据预计表达蛋白的分子量，可筛选阳性表达的重组体。 二、试剂准备 1、30%储备胶溶液：丙烯酰胺（Acr）29. ...

TITLE: Facilities for Aseptic Surgery SCOPE: All Animal Program Personnel RESPONSIBILITY: Facility Manager Surgical Core Manager All Animal Program Personnel PURPOSE: To Identify the Available Surgica ...

TITLE: Pest Control SCOPE: All Animal Program Personnel RESPONSIBILITY: Facility Manager Surgical Core Manager All Animal Program Personnel PURPOSE: To Outline the Program of Pest Control Within An ...

TITLE: Animal Quarantine Stabilization and Separation SCOPE: All Animal Program Personnel RESPONSIBILITY: Veterinary Staff Facility Manager Surgery Core Manager All Animal Personnel PURPOSE: To Out ...

TITLE: Animal Health and Environmental Surveillance SCOPE: All Animal Program Personnel RESPONSIBILITY: Facility Manager Surgical Core Manager All Animal Program Personnel PURPOSE: To Daily Review Ani ...

TITLE: Transportation Relocation or Reassignment of Animals SCOPE: All Animal Program Personnel RESPONSIBILITY: Facility Manager All Animal Program Personnel PURPOSE: To Outline the Proper Procedur ...

TITLE: Test and Control Articles SCOPE: All Animal Program Personnel RESPONSIBILITY: Facility Manager Surgical Core Manager All Animal Program Personnel Study Sponsor PURPOSE: To Outline the Methods ...

TITLE: Clinical Laboratory Sample Testing SCOPE: All Animal Program Personnel RESPONSIBILITY: Facility Manager Surgical Core Manager All Animal Program Personnel PURPOSE: To Outline the Procedures ...

TITLE: Handling Storage and Retrieval of Records and Data SCOPE: All Animal Program Personnel RESPONSIBILITY: Facility Manager Surgical Core Manager All Animal Program Personnel PURPOSE: To Outline ...

TITLE: Reagent Labeling SCOPE: All Animal Program Personnel RESPONSIBILITY: Facility Manager Surgical Core Manager All Animal Program Personnel PURPOSE: To Ensure Uniformity of Labeling of All Regents ...

TITLE: Animal Medical Records for Nonrodent Mammals SCOPE: All Animal Program Personnel RESPONSIBILITY: Clinical Veterinarians Facility Manager Veterinary Care Managerall Research and Animal Personnel ...

TITLE: Employee Orientation and Training SCOPE: All Animal Program Personnel RESPONSIBILITY: Facility Manager Surgical Core Manager All Animal Program Personnel PURPOSE: To Outline the Proper Proce ...

TITLE: Controlled Substances SCOPE: Research and Animal Program Personnel RESPONSIBILITY: Program Coordinator Veterinary Care Manager Facility Managers and All Animal Program Personnel URPOSE: To O ...

TITLE: Animal Identification SCOPE: All Animal Program Personnel RESPONSIBILITY: Program Assistant Facility Manager all Research and Animal Personnel PURPOSE: To Outline the Procedures for the Assignm ...

一、材料与仪器 30%丙烯酰胺溶液；1.5mol/L Tris-HCl分离胶缓冲液，PH8.8；1.0mol/L Tris-HCl浓缩胶缓冲液，PH6.8；电泳缓冲液，PH8.3；10%SDS溶液；10%过硫酸铵溶液；样品处理液；染色液；脱色液；电泳玻璃板，电泳电源架，电泳槽，电泳仪等；蛋白Mark。 二、方法与步骤 1) 分离胶和浓缩胶配制 按下表比例分别配制分离胶和浓缩胶： 2)凝胶板的准备 ...

TITLE: Animal Room Preparation SCOPE: All Authorized Personnel RESPONSIBILITY: Facility Manager and Technical Staff PURPOSE: To Outline the Proper Procedures for Animal Room Preparation 点击下面的链接下载全文： & ...