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细菌基因组DNA的提取方法综述，提供了5种方法。 1 快速微量提取法A.取1.5ml菌体培养物于一灭菌Ep管中，12000rpm离心1min 丢去上清夜，收集菌体。B.加入400ul裂解液（40mMTris-醋酸，20mM醋酸钠1mMEDTA1%SDSpH7.8）混匀置于37oC水浴1hr。C.然后加入200ul5mol/L的氯化钠溶液，混匀后于13000rpm离心15min。D.取上清液，用苯酚 ...

一、目的 掌握植物基因组DNA提取的一般方法及注意事项。大分子量DNA分子的酶切分析。二、原理十六烷基三乙基溴化胺是一种去污剂，可溶解细胞膜，它能与核酸形成复合物，在高盐溶液中（0.7mol/L NaCl）是可溶的，当降低溶液盐浓度到一定程度(0.3 mol/L NaCl)时，从溶液中沉淀，通过离心就可将CTAB与核酸的复合物同蛋白质、多糖类物质分开，然后将CTAB与核酸的复合物沉淀溶解于高盐溶液 ...

DNA Extraction from Archival Formalin-fixed Paraffin-embedded Tissue SectionsAuthor: Shi et al. Source: Contributed by APostodoc Abstract: Describes two methods of extracting DNA from archived paraffi ...

TA CloningI. Initial mixture5 μl dd H2O1 μl PCR product1 μl ligation buffer2 μl TA vector (add second to last)1 μl ligase (add last)Incubate overnight at 15℃.II. Electroporation1. Chill ...

PurposeDNA extraction without phenol extraction and centrifugation.Procedure1. Lysis: the lysis buffer (usually 0.5ml) is added to the tissue or cell ( for a 75cm2 flask add 5ml directly to the cell). ...

1.Grind in mortar and pestle or Waring blender with 5-7 volumes buffer A per g tissue. Use MCE at 350 l/L and if necessary with 5 ml 1 M DIECA/L. 2.Squeeze through cheesecloth two layers of Miracloth. ...

This method is derived from a procedure developed by Toby Bradshaw and the Poplar Molecular Genetics Cooperative. We have tested the procedure with a variety of Populus species as well as tobacco and ...

Purpose: Purification of oligonucleotides (which have already been purified by reverse phase) can increase the efficiency of site directed mutagenesis from around 30% to ~75% or greater in some cases ...

PrincipleThe nucleic acids contained within the gel slice are electrophoresed out of the gel funneled down into the v-shaped channel and trapped within a high salt cushion resting at the bottom of the ...

Sample Preparation Cheek cells are obtained by rinsing the mouth with 25mls of any commercial mouth wash solution available for about 30sec the first thing in the morning. It is important not to brush ...

Materials:Silica Suspension:add 2 g of silica to 15 ml of H2Owash 3x by centifugation at 2000 x g for 2 minestimate vol of silica and resuspend in 2 vol H2OSilica Wash Solution:50 mM NaCl 10 mM Tris 7 ...

- make a 5µm section to do an evaluation of the % tumour cells- make 50X50µm sections for the DNA isolation- put sections in a falcon tube containing 10 ml of 1XSE- add 100µg/ml prot. K (endconcentrat ...

Electroelution of agarose fragmentsElectroelution buffer1 M Tris pH 7.5 12.0 mls0.5 M EDTA 0.24 mls1 M NaCl 3.0 mlsqs to 600 mls dH2OAcetate cushion 3 M NaAcetate pH 4.8 480 μl0.1 % Bromphenol Blue ...

比起其它公司的原核表达系列来说Invitrogen有个非常突出的地方就是它的重组克隆技术。像之前Novagen大部分载体都是用传统的酶切、链接方式做重组质粒，但是Invitrogen的表达系统大量运用了它的TOPO技术和Gateway技术。虽然现在在许多做科研的学生看来，能出实验结果是最重要的，中间的过程不是那么重要。可是只有创新的技术才能推动科学不断的进步，比如：如果我们一直采用手工法测序，那真 ...

Procedure 1. Grind 2 to 5 g of frozen leaves to a very fine powder with a liquid nitrogen-cooled mortar and pestle.2. Add 25 ml of CTAB Buffer and transfer to a 50 ml tube.3. Incubate at 65℃ for 20 ...

Isolation of DNA from Mouse Tail Biopsies 1. Obtain tail biopsies from 2 to 3 week old mice: Hold mouse firmly at base of tail with one hand with the other cut off 0.5 to 1.0 cm of the tail tip with a ...

Phenol (removes protein) 1.add equal volume of Phenol (= tris-saturated Phenol-Chloroform-Isoamyethanol) 2.vortex 3.spin 2 minutes at 12000 rpm 4℃4.transfer supernatant to a fresh tube (avoid aspirati ...

1. Run gel (0.8 -1.0% agarose is best). For yeast chromosomal Southerns digest 20 μg DNA. Use 50 ml minigel for most purposes. Photograph gel but minimize exposure to UV light.2. Depurination: Place g ...

Protocol for Isolation of Genomic DNA from Mammalian Cells1.Trypsinize harvest and resuspend cells at 107 / ml in 10 mM Tris-HCl (pH 8.0) 10 mM EDTA. 2.Add SDS and Proteinase K to a final concentratio ...

Below we present the protocols we have used to isolate DNA from various tissues using Silica and Guanidinium Thiocyanate.These protocols are adapted from Boom et al. (1990)Höss & Pääbo (1993)and Höss ...