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验室有毒物质介绍和防护注意事项申请加分乙酸(浓)必须非常小心地操作。可能由于吸入或皮肤吸收而受到伤害。要戴合适的手套和护目镜。在化学通风橱\生物安全柜里使用。·乙腈是非常易挥发和特别易燃的，它是一种刺激物和化学窒息剂，可因吸入、咽下或皮肤吸收而发挥其效应。严重中毒的病人可按氰化物中毒方式处理。操作时要戴合适的手套和安全眼镜。只能在通风橱\生物安全柜里使用，远离热、火花和明火。·丙烯酰胺(未聚合的)是一种强的神经毒剂并 ...

ftp上的pdquest，下载后才知道是只能用basic mode，很多功能是受到限制的，不知道哪位大侠有它的patch，供大家共享呀！此外，有pdquest的教学东东，申请上传！大概有180M。下面是一个简介：PDQUEST Tutorial Read MeIf the CD-ROM fails to auto-start, open the root directory of the CD using Windows Explorer and double-click on the RunTutori ...

J AM SOC NEPHROL7.689EUR UROL7.667KIDNEY INT6.193AM J KIDNEY DIS5.152J SEX MED4.884CLIN J AM SOC NEPHRO4.884J UROLOGY4.016CURR OPIN NEPHROL HY3.961AM J PHYSIOL-RENAL3.731AM J NEPHROL3.481SEMIN DIALYSIS3.366NEPHROL DIAL TRANSPL3.306PROSTATE3.081BJU INT2.865SEM ...

今天进行了GST融合蛋白的表达，pGEX-4T-1载体,BL21菌。步骤：摇菌至A600：0.7，然后加IPTG诱导3小时，温度35度（终浓度：0.3mM)，取1ml诱导后菌，离心去培养基，冰冷PBS洗一次，离心，吸净液体，加入undefined上样缓冲液50微升和终浓度1mM PMSF，100度煮菌5分钟，离心，取20微升上清跑胶。结果如图。1.空载体对照有GST的表达2.所有重组载体同样有GST大小的片断，（箭头 B）3.箭头A所示的条带在空载体 ...

Heparin-Sepharose Precipitation and ImmunoblottingTo analyze for CTGF protein expression, conditioned media were collected and the heparin-binding proteins were precipitated by end-over-end mixing for 4 h at 4°C with heparin-Sepharose CL-6B beads from Pharmacia (Piscata ...

09新书：Food Chemistry,4ed. PDFH.-D. Belitz, Werner Grosch, Peter Schieberle "Food Chemistry,4 Ed."Springer | 2009-02-01 | ISBN: 3540699333 | 1117 pages | PDF | 11,5 MBFor more than two decades, this work has remained the leading advanced textbook and easy-to-use reference on food chemistry and te ...

本内容需要回复后才能下载！本书版权归作者所有，请于下载后24小时内删除图解生物化学（英文版）Harper's Illustrated Biochemistry(26thAnnotation UP TO DATE, SUCCINCT, AND MEDICALLY RELEVANTHarper's Biochemistry offers concise yet authoritative coverage of the principles of biochemistry and molecul ...

Publisher: CRCNumber Of Pages: 768Publication Date: 2005-07-27Sales Rank: 614384ISBN / ASIN: 0824754662EAN: 9780824754662Binding: HardcoverManufacturer: CRCStudio: CRCAverage Rating: 5Total Reviews: 1--------------------------------------------------------------------------------Book D ...

Top 100 proteomics publications----From Ion Channel Media Group：ttp://www.ionchannelmedia.com/websites.php1. Gavin et al. (2006) Proteome survey reveals modularity of the yeastcell machinery. Nature2. Bonetta et al. (2006) Protein purification: proteomics at Harvard. Na ...

刚刚从小木虫上看到，还不错，和大家一起分享：Book DescriptionAntibodies are an indispensable tool in the study of biology and medicine. Making and Using Antibodies: A Practical Handbook presents techniques in a single, comprehensive source for the production and use of antibodies. It enables resea ...

我看很多朋友问到酸性电泳的问题，现在提供我的一个配方，希望对大家有用！酸性非变性电泳工作液配制：40%胶贮液(Acr:Bis=29：1）4×分离胶Buf(pH4.3,0.24M KOH-HAc)：1.346g KOH, 以HAc 调pH至4.3，Milli-Q定容至100ml。4×堆积胶Buf(pH6.8,0.24M KOH-HAc)：1.346g KOH, 以HAc 调pH至6.8，Milli-Q定容至100ml。5×电泳Buf（pH4.4,β-Ala-HCl）：35.6 ...

上周送了一个考染的蛋白质点做PMF质谱鉴定，我自认为点取准了，质谱的蛋白量足够，可公司的人说我送去的样品太少，PMF做不出来，但是可以做MALDI-TOF-TOF。没办法，做了一个月的实验不能白做吧！狠下心多加了200元做了MALDI-TOF-TOF。（鉴定一个蛋白质的钱可以买一台三星的17寸显示器了）结果如下：Accession Mass Score Description1. gi|34903780 51933 67 unnamed prot ...

Purification proteins by TALON50 ml Cells weight (mg) Bugbuster (ml) 1´Extraction buffer (ml) TALON (ml) Resin (ml) Elution buffer (ml)300 2.0 2.0 1000 500 2502.0 ml eppendorf tubes should be used for mini-purification. For this purpose, if the total volume is more than 2 ml, try to use 5´Extraction buff ...

我下了几个学习专业术语的mp3，不过，太大了，不好上传，是podcast。http://www.podcastdirectory.com 是一个很好的分享podcast的网站。我给大家介绍的这个网站网址在附件里面。整天帮我同学下东西，我的丁当不够用，别嫌我不厚道。:DWhat is Glycomics? What is new in the world of science? What’s out there that can impact humanity? Why haven’t I heard of it? ...

今天早上收到一封邮件，这一期的JPR是免费的？呵呵 ：）Special Issue: Liver Proteomics – Also the 2010 Journal of Proteome Research Sample IssuePlease take a moment to browse through this free sample issue to investigate the broad coverage of the field available in a given issue of the Journal of Proteome Research. ...

wiley出版共10章Chapter 1:Protein Microarrays: From Fundamental Screening to Clinical Diagnostics (p 1-9)Chapter 2:Protein Microarray Surface Chemistry and Coupling Schemes (p 11-38)Chapter 3:Optimization of a Protein Microarray Platform Based on a Small-molecule Chemi ...

这是一个值得骄傲的中国人的工作，第一块真核生物全蛋白组芯片就出自他手，下面是他近来发表的文章：1. *H Zhu（Heng Zhu）, JF Klemic, S Chang, P Bertone, A Casamayor, KG Klemic, D Smith, M Gerstein, MA Reed, M Snyder. Analysis of yeast protein kinases using protein chips. Nat. Genet. 26: 283 -9 (2000).2. *H Zhu , M Bilgin, M Snyder. Proteomics Ave. Rev. Bioc ...

有几个资料网址，请大家分享：标题：NSTL订购的国外网络版期刊,面向中国大陆学术界用户开放。国家科技图书文献中心（ NSTL ）新近订购了外文网络版全文期刊，并达成了全国授权协议，中国大陆地区的学者可以免费在线阅读全文电子期刊。对于NSTL73种期刊，前五家学会的镜像文件都放在了国内服务器上，浏览不需要国际流量。请登陆 http://www.nstl.gov.cn/htm/qwwx/index.jsp 获取详细信息。1. 加拿大国家科学委员 ...

Lysosomes (Medical Intelligence Unit)溶酶体Lysosomes (Medical Intelligence Unit~undefined Publisher: Springeundefined Number Of Pages: 20undefined Publication Date: 2005-08-0undefined Sales Rank: 165527undefined ISBN / ASIN: 038725562undefined EAN: 978038725562undefined Binding: Hardcoveundefined Manufacturer: Springeundefined Studio: S ...

后基因组时代的蛋白质结构与功能研究随着"人类基因组计划"的迅速进展以及包括水稻等30多种多种生物的基因组被完全测序，我们已经大步跨入了后基因组时代。在后基因组时代要回答和解决的众多问题中， 对这些基因编码的蛋白质的研究更显得重要和迫切。结构基因组学 （Structural Genomics）: 用大规模、高通量的方法测定尽可能多的蛋白质三维结构。研究的着重点不是所有的蛋白质，而是每种蛋白质家族的代表性结构域，因为结构域是蛋白 ...