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Contents:Table of ContentsAdhesion Protein ProtocolsPrefaceContentsContributors1— Monoclonal Antibodies Specific for Leukocyte Adhesion Molecules Selective Protocols of Immunizati...1— Introduction2— Materials3— Methods4— NotesReferences2— Epito ...

TCA-DOCFor precipitation of very low protein concentration1) To one volume of protein solution, add 1/100 vol. of 2% DOC (Na deoxycholate, detergent).2) Vortex and let sit for 30min at 4ºC.3) Add 1/10 of Trichloroacetic acid (TCA) 100% vortex and let sit ON at 4ºC (preparation of 100% TCA: 454ml H2O/kg TCA. Ma ...

注册了半天，终于搞下来了！http://www.bio-rad.com/cmc_upload/Literature/202685/Install_PDQuest_v801_PC.zipThe download version of PDQuest has a free 30 day trial period, during which you do not normally need an HSK. After the trial period expires, you can continue to use PDQuest in Viewer mo ...

通过对90多种真核蛋白在原核中的表达总结出提高可溶表达的一些经验，很有借鉴意义～AbstractBackground: In the search for generic expression strategies for mammalian protein families several bacterial expression vectors were examined for their ability to promote high yields of soluble protein. Proteins ...

看了结果却不太明白，究竟有没有跨膜区？能不能做原核表达？谢谢TMpred output for unknown Date: Wed Jul 28 16:50:41 Europe/Zurich 2004--------------------------------------------------------------------------------Sequence: MSS...NNS, length: 431Prediction parameters: TM-helix length between 17 and 33---------------------------------------- ...

Publisher: Wiley-VCHNumber Of Pages: 304Publication Date: 2006-01-09Sales Rank: 488335ISBN / ASIN: 3527308199EAN: 9783527308194Binding: HardcoverManufacturer: Wiley-VCHStudio: Wiley-VCHAverage Rating:Total Reviews:--------------------------------------------------------- ...

Glycoprotein Analysis in BiomedicineGlycoprotein Analysis in Biomedicine(Methods in Molecular Biology)Glycoprotein Analysis in Biomedicine brings together a comprehensive range of protocols related to carbohydrate biochemistry. The first half of the manual outl ...

Publisher: CRCNumber Of Pages: 400Publication Date: 2006-02-23Sales Rank: 871877ISBN / ASIN: 157444526XEAN: 9781574445268Binding: HardcoverManufacturer: CRCStudio: CRCAverage Rating:Total Reviews:--------------------------------------------------------------------------------Book D ...

偶然发现的一个pQE30系统表达蛋白纯化案例，帖出来和大家分享。Purification of LF.诱导与表达E. coli SG13009(pREP4) carrying the recombinant plasmid pPG-LF1 was grown at 37°C in Luria broth with 100 μg of ampicillin and 25 μg of kanamycin per ml at 250 rpm.When the A600 reached 1.0, isopropyl-1-thio-β-d-galactopyran ...

SOUTHERN BLOT1. Run the gel as normal. Often for genomic southerns it is desirable to run long gels (18cm) over 4-6hrs.2. Photograph the gel with a ruler adjacent to the molecular weight markers as a reference.3. Alkali transfer buffer = 0.5M NaOH (20g/lt), 1.5M NaCl (87.66 g/lt). Prepare 1 litre for one gel and an ...

近期查找蛋白质组相关资源，今对丁香园部分相关资料做了个整理链接，以便大家查阅：蛋白质组研究分析中心（RCPA）－－蛋白质组研究网（由huang3301发贴）http://www.proteomics.ac.cn/cindex.html蛋白质组学必备——网站推荐（riversider于2005-04-03发贴）http://www.biochem.oulu.fi/proteomics/protocols.html蛋白质组学绪论（p ...

题目：Data_Analysis_and_Visualization_in_Genomics_and_Proteomics编者：Francisco Azuaje (Editor), Joaquin Dopazo (Editor)ISBN: 0-470-09439-7Hardcover 284 pages May 2005格式：PDF大小：2.41MB原书连接：http://as.wiley.com/WileyCDA/WileyTitle/productCd-047 ...

Chemical and Functional Properties of Food SaccharidesPiotr TomasikPublisher: CRCNumber Of Pages: 440Publication Date: 2003-10-20Description:With a broad spectrum of knowledgeable sources, numerous tables, and figures identifying structures and reactions, and the ...

Gelatin Zymography Protocol1.Prepare gels (7.5%, 10% or 12%) according to the standard procedure. When preparing the running gel add gelatin stock solution (10 mg/ml in H2O) to get the gelatin concentration of 0.1% (1 mg/ml).2.Mix one part sample with one part Tris-Glycine SDS Sample Buffer (2x) and let ...

本内容需要回复后才能下载！本书版权归作者所有，请于下载后24小时内删除真实的蛋白质色谱（高清英文版）Practical Protein Chromatography名称Practical Protein ChromatographyBiological methodsMethods in Molecular Biology第 第 11 卷 卷, Jeffrey W. Pollard编者Andrew Kenney, Susan Fowell版本插图版出版商Humana Press, 1 ...

这个地址里有很多蛋白纯化的手册,自己用E-MAIL注册一下!就可以下载了http://www4.amershambiosciences.com/aptrix/upp00919.nsf/content/list_all_handbooks2D Electrophoresis using immobilized pH gradients - Principles and MethodsTwo-dimensional electrophoresis (2-D ele ...

Publisher: Wiley-VCHNumber Of Pages: 393Publication Date: 2005-05-06Sales Rank: 1682711ISBN / ASIN: 3527308377EAN: 9783527308378Binding: HardcoverManufacturer: Wiley-VCHStudio: Wiley-VCHAverage Rating:Total Reviews:------------------------------------------------------- ...

信息1、Recombinant Gene Expression : Reviews and ProtocolsEditor(s): Paulina Balbás1, Argelia Lorence2Affiliation(s): (1),Centro de Investigación en Biotecnología,CuernavacaMéxico(2)Virginia Polytechnic Institute,State University,BlacksburgVASeri ...

09新书：The Septins .PDFPeter A. Hall (Editor), S. E. Hilary Russell (Editor), John R. Pringle (Editor)ISBN: 978-0-470-51969-1Hardcover380 pagesJanuary 2009Septins have recently attracted a strong interest form researchers of diverse fields of biological and medical sciences. A co ...

1.内容：Plant Genomics and Proteomics(英文)2.格式：PDF3.作者：Christopher A. Cullis4.大小：1.7MB5.出版社：2004 John Wiley & Sons, Inc.7.简介：见下Table of contentsFrontmatter (p i-xiv)Christopher A. CullisSummary | Full Text: PDF (Size: 72K)Chapter 1:The Structure of Plant Genomes (p 1-22)Chri ...