In Vivo Functional Analysis of Transcription Factor: Response Element Interaction Using Transgenic Xenopus laevis

Analysis of transcription factor–target interactions in vivo is important to the study of transcriptional regulation of gene expression. A key experiment involves analysis of the functional interaction between a trans -acting factor and its corresponding cis -acting element in the context of a target promoter in vivo. We describe a method for this analysis in transgenic Xenopus tadpoles in which expression of the trans -acting factor is knocked down using an shRNA-mediated approach.