Analysis of In Vivo Occupancy of Aebp1, a Transcription Factor, Using High Resolution Tiling Array

Aebp1 is a transcription factor that negatively regulates adipogenesis and also is upregulated in primary GBM as against secondary GBM. Here we describe a systematic approach to map the in vivo occupancy sites of Aebp1 by using a combination of chromatin immune-precipitation technique and hybridization to Agilent high resolution human promoter tiling array (2�224) containing 474393 probes. We also describe the use of various bioinformatics tools for analysis of the hybridization data and identification of Aebp1 occupancy sites in U87MG glioma cell line.