PCR-Based Cloning of Cortically Localized RNAs from Xenopus Oocytes

RNA localization is an important cellular mechanism for restricting the activities of regulatory proteins to cytoplasmic domains in oocytes and embryos (1 ). Cloning of these localized RNAs is obviously the first step in determining the functions of the encoded proteins. However, localized RNAs are rare and in low abundance. Their efficient isolation requires a combination of procedures to enrich for these RNAs.