E.Z.N.A.TM Plant DNA Maxi Protocol For Dry Specimens
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实验试剂
1. Equilibrate Elution Buffer or 10 mM Tris pH 9.0 at 65°C.
2. Isopropyl alcohol (isopropanol)
3. Absolute (96%-100%) ethanol
实验设备
1. High speed centrifuge capable of at least 10,000 x g
2. Centrifuge with swinging-bucket rotor at room temperature capable of 4000xg.
4. Waterbath equilibrated to 65°C
实验步骤
2. Incubate at 65°C for 30-60 min. Mix sample by vortexing during incubation.
5. Centrifuge at 10,000 x g for 15 min to pellet DNA. Longer centrifugation does