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        Assessing Antigen-Specific CD8+ and CD4+ T-Cell Responses in Mice After Immunization with Recombinant Viruses

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        To determine the immunogenicity of vaccine candidates, it is essential to establish an accurate and convenient methodology for assessing the level of antigen-specific T-cell responses, and several methodologies have been developed. These include the T-cell proliferative assay as determined by tritium-labeled thymidine (3 H-TdR) incorporation, cytotoxic assays such as the chromium release assay, cytokine enzyme-linked immunosorbent assays (ELISAs) or the T-cell enzyme-linked immunospot (ELISPOT) assay to determine the amounts of cytokines released by activated T cells, and most recently, the newly developed tetramer assay. All these methods, each of which have both advantages and disadvantages, have been used to measure the level of cell-mediated immune responses in animals and humans infected with microbes or immunized with microbial antigens or various vaccines.
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