Blank control (Black line): HUVEC(Black).
Primary Antibody (green line): Rabbit Anti-von Willebrand antigen 2 antibody (bs-4754R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
- Bioss
- bs-4754R
- 2025年10月16日
- 产品信息以Bioss网站为准
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-4754R |
| 英文名称 | VWF Rabbit pAb |
| 中文名称 | 血管假性血友病因子/血管性血友病因子抗体 |
| 英文别名 | Coagulation factor VIII; F8VWF; Factor VIII related antigen; von Willebrand antigen 2; Von Willebrand antigen II; Von Willebrand disease; Von Willebrand Factor; VWD; VWF_HUMAN. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=3ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Dog, Pig, Cow, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Von Willebrand Factor |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 81/309 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Invasion/microenvironment > Angiogenesis > Angiogenic growth factors Cardiovascular > Angiogenesis > Endothelial Cell Markers Cardiovascular > Blood > Coagulation > Extrinsic Cardiovascular > Blood > Coagulation > Regulatory Cardiovascular > Blood > Platelets Cardiovascular > Blood > Serum Proteins Stem Cells > Endothelial Progenitors > Endothelial Markers |
| 亚基 | Multimeric. Interacts with F8. |
| 亚细胞定位 | Secreted. Secreted, extracellular space, extracellular matrix. Note=Localized to storage granules. |
| 组织特异性 | Plasma. |
| 翻译后修饰 | All cysteine residues are involved in intrachain or interchain disulfide bonds. N- and O-glycosylated. |
| 相似性 | Contains 1 CTCK (C-terminal cystine knot-like) domain. Contains 4 TIL (trypsin inhibitory-like) domains. Contains 3 VWFA domains. |
| 功能 | Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Von Willebrand Factor (VWF) was previously known as Factor VIII related antigen. VWF is synthesized exclusively by endothelial cells and megakaryocytes, and stored in the intracellular granules or constitutively secreted into plasma. This glycoprotein functions as both an antihemophilic factor carrier and a platelet vessel wall mediator in the blood coagulation system. Important in the maintenance of homeostasis, it participates in platelet vessel wall interactions by forming a noncovalent complex with coagulation factor VIII at the site of vascular injury. The Von Willebrand factor has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. Mutations in this gene or deficiencies in this protein result in Von Willebrand's disease. VWD is characterized by frequent bleeding (gingival, minor skin quantitative lacerations, menorrhagia, etc.). |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {3ug/Test} |
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (von Willebrand antigen 2) Polyclonal Antibody, Unconjugated (bs-4754R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (von Willebrand antigen 2) Polyclonal Antibody, Unconjugated (bs-4754R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验该产品被引用文献
[IF={{ 7.3 }}] {Shi Xin. et al. The bone marrow endothelial progenitor cell response to septic infection. FRONT IMMUNOL. 2024 Apr;15:} {IF} {Mouse}
[IF={{ 4.8 }}] {Ting Shu. et al. Sensitive electrochemical detection of von Willebrand factor using an immunosensor comprising 7,7,8,8-tetracyanoquinodimethane and nickel-based metal–organic framework. MICROCHEM J. 2023 Sep;:109351} {Other} {Human}
[IF={{ 2.73 }}] {Xenia Kraus. et al. A pre-conditioning protocol of peripheral blood derived endothelial colony forming cells for endothelialization of tissue engineered constructs. Microvasc Res. 2021 Mar;134:104107} {IHC} {Human}
[IF={{ 2 }}] {Atsuo Fujito. et al. The Mechanism of 5-Fluorouracil-Induced Hyperpigmentation in HRM-2 Hairless Mice: Focus on the Increase of Blood Vessels. BIOL PHARM BULL. 2024 Jan;47(1):311-317} {IHC} {Mouse}
[IF={{ 17.694 }}] {Tanaka Miwa. et al. ASPSCR1::TFE3 orchestrates the angiogenic program of alveolar soft part sarcoma. NAT COMMUN. 2023 Apr;14(1):1-16} {WB} {Mouse}
相关实验
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
Evaluation of Immunogenicity of Rat ES-Cell Derived Endothelial Cells
rat endothelial cells (EC). In a first characterization step, the endothelial nature of rat ES cell derived endothelial cells was proved by labelling with von Willebrand factor (vWF) as well as testing tube formation capacity on an extracellular matrix
分子 -1 (rabbit ICAM-1) ELISA 48T/96T 2000/3000 进口分装 F20170 兔干扰素 -r(rabbit IFN-r) ELISA 48T/96T 1500/2500 进口分装 F20180 兔白介素
技术资料暂无技术资料 索取技术资料
文献支持
VWF Rabbit pAb(bs-4754R)-50ul/100ul/200ul
￥1180 - 2800
