Suppressor tRNAs: Protocols and Applications for Cardiac Gene Transfer

Although tRNA-mediated suppression has been mainly used to study chainterminating mutations in bacteria and yeast (1 ,2 ), suppressor tRNAs have also been employed for a variety of other purposes. For example, by introducing a nonsense mutation into the diphtheria toxin-coding sequence, its expression in vivo can be regulated by the presence or absence of a suppressor tRNA. This technique has provided new approaches to cancer therapy (3 ) as well as to the study of visual system development in Drosophila melanogaster (4 ).