Recombinant-Rhizobium-radiobac
ter-Conjugal-transfer-protein-trbCtrbCConjugal transfer protein trbC- ￥9968
- CUSABIO
- CN
- CSB-CF346990RKV
- 2025年07月14日
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- 文献和实验
- 技术资料
- 保存条件：
"-20°C/-80°C"
- 保质期：
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
- 英文名：
Customized Rhizobium radiobacter trbC Protein (in vitro E.coli)
- 库存：
200
- 供应商：
武汉华美生物工程有限公司
- 规格：
20ug
Alternative Name(s)
Conjugal transfer protein trbCEditorial/Sponsord
EditorialUniprot ID
P54908Gene Names
trbCOrganism
Rhizobium radiobacterAASequence
MSLKTHHTPIFTALALVALGSLDGALASSGGGSLPWESPLQQIQQSITGPVAGFIALAAV AIAGAMLIFGGELNDFARRLCYVALVGGVLLGATQIVALFGATGASIGELHSQVDPFGYS PSPKLIERGEGAHGExpression Region
1-134aaSequence Info
Full lengthSource
in vitro E.coliSource Notice
Mammalian cell expression systems and other species are available. Please inquire.Tag Info
InquireMW
InquireList Price
1424Purity
Greater than 85% as determined by SDS-PAGE.Storage Buffer
Tris/PBS-based buffer, 6% TrehaloseStorage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.Endotoxin
Not Test. Endotoxin removal service is available for free upon you request.产品类型
Transmembrane-Protein备注
**产品信息可能有变动，请以官网信息为准
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文献和实验**产品信息可能有变动，请以官网信息为准
, 0. 1mM 苯甲基磺酰氟(DMSF ) , 5mM B2巯基乙醇, 0. 5% CHA P s(Pierce) 和10% 甘油。 (3)冰育30 分, 然后在超速离心机中离心30 分钟。 (4)去上清, 液氮速冻沉淀, 裂解细胞于-80℃保存。 二、扩增 (1)在微量管中冻干0. 1ug 荧光标记的cx反转引物, 蜡封(5′CCC TTA CCC TTA CCC TTA CCC TTA 3′)。 (2)于蜡界之上加50ul TRA P 反应物, 包括T ris-Cl (pH8. 3) 20
Gene Transfer and Expression of Recombinant Proteins in Moderately Halophilic Bacteria
Pyrococcus woesei . Vector pHS15, which was designed for expression of heterologous proteins in both E. coli and MHB, was used for the subcloning and transfer of the above genes. The recombinant constructs were introduced to MHB by assisted conjugal transfer
增殖时可把 F 因子传递给后代；（ 2 ）没有 F 因子的细菌称为 F － ， F ＋ 细菌经 吖 啶橙处理而丢失，成为 F － 。 F 因子一经丢失，细胞中便不再出现；（ 3 ） F ＋ 可以和 F － 杂交，而不能和 F ＋ 杂交；（ 4 ） F ＋ × F － 的杂交后代皆为 F ＋ ，而且可以 10 － 7 频率获得重组体后代。 F 因子介导的接合也和滚环复制有关。其转移区（ transfer
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