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        PCR佐剂

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        1706

         

        <center> <table> <tbody> <tr> <td> <p>  </p> <font color="#af0000"><font>Various authors recommend DMSO and glycerol to improve amplification efficiency (higher amount of product) and specificity (no unspecific products) of PCR, when used in concentrations varying between 5-10% (v/v). In the multiplex reaction, however, these adjuvants gave conflicting results. For example, 5% <b>DMSO</b> (Fig. 42) improved the amplification of some products, decreased the amount of others whereas some loci were not influenced at all. Similar results were obtained with 5% <b>glycerol</b> (data not shown). Therefore, the usefulness of these adjuvants needs to be tested in each case.</font> </font> <p>  </p> <p> <font color="#af0000"><font><b>BSA</b> , in concentrations of up to 0.8µg/µl (higher than previously described) appeared to increase the efficiency of the PCR reaction much more than either DMSO or glycerol. It should be noted that BSA did not have an inhibitory effect on any of the loci amplified (data not shown).</font> </font></p> </td> </tr> <tr> <td> <p> </p> </td> <td> <p> <font color="#5247f1"><b><font>Fig. 42.</font> </b> <font> Comparative multiplex PCR using mixtures A to D with 5% DMSO (superscript D) and without DMSO, in 1x buffer. Some loci from mixture A (blue arrows) are stronger when no DMSO is used. However, DMSO helps amplify (magenta arrows) one locus in mix B and one locus in mixture D. Amplification of PCR products of mixture undefined were unaffected by DMSO.</font> </font></p> </td> </tr> </tbody> </table> </center>

        PCR方法相关产品:

        • 电泳设备
        • 紫外设备
        • 普通PCR仪
        • 定量PCR仪
        • PCR/RT-PCR/qPCR试剂
        • PCR引物
        • PCR试剂
        • PCR对照
        • 特异性PCR试剂盒
        • PCR克隆试剂盒
        • RNA
        • RNase检测/去除
        • RT-PCR试剂
        • RT-PCR标准品
        • 定量PCR试剂
        • 定量PCR标记
        • 总RNA分离纯化盒
        • PCR产物纯化
        • 核酸酶
        • 聚合酶
        • 反转录酶

         

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