HLA-DR基因中等分辨率分型序列特异性引物
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HLA-DR 基因中等分辨率分型序列特异性引物
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5’-引物
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序列(5’ � 3’)
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3’-引物
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序列(5’ � 3’)
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PCR产物长度
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DRB1基因扩增特异性
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5’01
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TTGTGGCAGCTTAAGTTTGAAT
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3’047
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CTGCACTGTGAAGCTCGCAC
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255bp
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0101-0102
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3’048
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CTGCACACTGTGAAGCTCTCCA
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255bp
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|
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5’01
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TTGTGGCAGCTTAAGTTTGAAT
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3’10
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CCCGCTCGTCTTCCAGGAT
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130bp
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0103
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5’02
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tcctgtggcagcctaagag
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3’01
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CCGCGCCTGCTCCAGGATT
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197bp
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1501,1502
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5’02
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tcctgtggcagcctaagag
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3’03
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AGGTGTCCACCGCGGCG
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213bp
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1601,1602
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5’03
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TACTTCCATAACCAGGAGGAGA
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3’03
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TGCAGTAGTTGTCCACCCG
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151bp
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0301,0302
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5’06
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GACGGAGCCGGTGCGTA
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3’48
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CTGCACACTGTGAAGCTCTCCA
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217bp
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0301
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5’03
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TACTTCCATAACCAGGAGGAGA
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3’47
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CTGCACTGTGAAGCTCGCAC
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189bp
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0302,1302,1305
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|
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1402,1403,1409
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5’04
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GTTTCTTGGAGCAGGTTAAACA
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3’47
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CTGCACTGTGAAGCTCGCAC
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260bp
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0401 -0411
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|
|
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3’048
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CTGCACACTGTGAAGCTCTCCA
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260bp
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|
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5’07
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CCTGTGGCAGGGTAAGTATA
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3’079
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CCCGTAGTTGTGTCTCGACAC
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232bp
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0701,0702
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5’08
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ACTACTCTACGGGTGAGTGTT
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3’05
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CTGCAGTAGGTGTCCACCAG
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214bp
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0801-0806
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5’09
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gtttcttgaagcaggataagttt
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3’079
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CCCGTAGTTGTGTCTCGACAC
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236bp
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0901
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5’10
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gttcttggagtactctacgtc
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3’047
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CTGCACTGTGAAGCTCGCAC
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204bp
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1001
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5’05
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gttCTTGGAGTACTCTACGTC
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3’06
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CTGGCTGTTCCAGTACTCCT
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176bp
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1101 - 1105
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5’08
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ACTACTCTACGGGTGAGTGTT
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3’08C
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TCTGTGAAGCTCTCCACAG
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248bp
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1201,1202
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5’03
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TACTTCCATAACCAGGAGGAGA
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3’10
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CCCGCTCGTCTTCCAGGAT
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130bp
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1301,1302
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5’05
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gttCTTGGAGTACTCTACGTC
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3’11
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TCTGCAATAGGTGTCCACCT
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224bp
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1401,1404,1405
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5’08
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ACTACTCTACGGGTGAGTGTT
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|
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1407,1408
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5’03
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TACTTCCATAACCAGGAGGAGA
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3’12
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TCCACCGCGGCCCTCC
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140bp
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1402,1404,1409
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|
|
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1305,1306
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5’04
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GTTTCTTGGAGCAGGTTAAACA
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3’19
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ctgttccagtctccgcag
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188bp
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1410
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5’52.1
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TTTCTTGGAGCTGCGTAAGTC
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3’13
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CTGTTCCAGGATCGGGCGA
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171bp
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DRBundefined0101 - 0301
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|
5’52.2
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GTTTCTTGGAGCTGCTTAAG
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3’14
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GCTGTTCCAGTACTCGGCAT
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173bp
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|
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5’53
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GAGCGAGTGTGGACCTGA
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3’048
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CTGCACACTGTGAAGCTCTCCA
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213bp
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DRBundefined0101
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|
5’51
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GTTTCTTGCAGCCAGGATAAGTA
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3’01
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CCGCGCCTGCTCCAGGATT
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198bp
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DRBundefined0101 �0 202
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|
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3’16
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CCGCGGCGGCCTGTCT
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207bp
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