Combining Site-Specific Chemical Modification with Site-Directed Mutagenesis: Versatile Strategy to Move Beyond Structural Limitations of 20 Natural A
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The invention of site-directed amino acid (AA) mutagenesis (1
) has revolutionized the way in which enzyme mechanism and specificity can be probed, while simultaneously providing a route
for engineering novel enzyme properties. Prior to this very precise method of AA replacement, protein chemists utilized chemical
modification and chemical mutagenesis techniques to alter protein and enzyme properties (2
–4
).
