Triplex-Directed Site-Specific Genome Modification

The ability to target and manipulate specific mammalian genes has been a long sought goal in biotechnology and biomedicine. The realization of this goal has only recently become feasible, with advances in genetic engineering. Through the use of gene targeting strategies, it is possible to replace genetic information on the chromosome, delete sequences from the chromosome, create transgenic mice, and develop new approaches to gene therapy of human genetic diseases (1 ).