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An effective method of completely removing contaminating genomic DNA from an RNA sample to be used for PCR

互联网

487
A simple method for removing contaminating genomic DNA from an RNA preparation is presented. The method involves digestion of the RNA with RNase-free DNase I at room temperature followed by inactivation of the enzyme at 65�C in presence of EDTA. This method produces an RNA sample that is negative for genomic DNA by PCR.
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