我要登录|
免费注册
|
我的丁香通
- 企业机构：
- 成为企业机构
- 个人用户：
- 个人中心
移动端

大家都在搜

大家都在搜

0 人通过求购买到了急需的产品

免费发布求购

点赞

收藏

分享

E.Z.N.A.® Mag-Bind® Viral DNARNA Spin Protocol

互联网2013-11-13

1466

实验原理

If using the E.Z.N.A.™ Mag-Bind^® Viral DNA/RNA Kit for the first time, please read this booklet to become familiar with the procedure and its various modification. Samples are lysed in a specially formulated buffer containing detergent. DNA/RNA was bound to the surface of Mag-Bind^® magnetic particles under proper condition. Proteins and cellular debris are efficiently washed with few wash steps. Pure RNA and DNA is then eluted in nuclease-free water or low ionic strength buffer. Purified RNA or DNA can be directly used in downstream applications without the need for further purification.

实验步骤

1. Distribute 150 ul sample into a 1.5 ml tube or a 96 deep well plate. If using pre-frozen samples, thaw at room temperature (15-20°C) and mix well by shaking or pipetting before proceeding step 2.

2. Add 300 ul of QVL Lysis Buffer, 6ul Glycogen and 20ul Mag-Bind^® Magnetic Beads R to the sample. Vortex at maxi speed for 30 seconds. Incubate at room temperature for 10 min.

Note: Add 20 ul 2-mercaptoethanol per 1 ml of QVL Lysis Buffer before use. This mixture can be made and stored at room temperature for 1 week.

3. Add 500ul Isopropanol followed with mix thoroughly by vortexing or pipetting up and down 30 times. Incubate at room temperature for 10 minutes.

4. For 1.5 ml tube, centrifuge at 10,000 x g for 5 min, or centrifuge at 4000 x g for 10 min for 96-well plate. Remove and discard the supernatant.

5. Add 0.5ml Buffer VHB to each sample. Vortex to completely resuspend magnetic paritcles.

Note: Buffer VHB must be diluted with absolute ethanol before use. Refer to label on bottle for instruction.

6. Centrifuge at 10,000 x g for 5 min for 1.5ml tube, or centrifuge at 4000 x g for 10 min for 96-well plate. Remove and discard the supernatant.

7. Add 0.7ml SPR Wash Buffer to each sample. Vortex to completely resuspend magnetic paritcles.

8. Centrifuge at 10,000 x g for 5 min for 1.5ml tube, or centrifuge at 4000 x g for 10 min for 96-well plate. Remove and discard the supernatant.

9. Repeat step 7-8 by adding another 0.7 ml SPR Wash Buffer to each sample.

10. Remove and discard the supernatant. Briefly spin to collect the liquid into the tube bottom. Remove any of liquid by pipettor and air dry 10-15 min.

11. Add 20-50ul nuclease free water, vortex to suspend magnetic beads completely. Incubate 3-5 minutes at room temperature. Centrifuge at 10000xg for 2 min, or centrifuge at 4,000 x g for 10 min. Transfer solution into a new tube or a new 300ul 96-well plate.

ad image

ad image

相关产品推荐

Bind-Silane 亲和硅烷

￥188

Recombinant-Acanthamoeba-polyphaga-mimivirus-Mitochondrial-carrier-like-protein-L276MIMIL276Mitochondrial carrier-like protein L276 Alternative name(s): VMC1 Viral mitochondrial carrier

￥10892

MAG/Siglec-4a重组蛋白|Recombinant Human MAG / GMA / Siglec-4 Protein (Fc Tag)

￥3870

Recombinant Human SPIN1/人源SPIN1蛋白

￥2870

免疫沉淀protocol

￥600

相关问答

问

大家是怎么溶解蕨素Z的呀

问

大家是怎么溶解蕨素Z的呀

问

普利莱E1015

4 回答 440 围观

相关方法

用SGM Plus®进行扩增

2024-05-14

Strep 标签蛋白纯化 protocol

2023-06-02

植物单细胞测序 protocol——水稻花序原生质体制备方法

2023-06-01

推荐阅读

E.Z.N.A.® Mag-Bind® Viral DNA/RNA Spin Protocol

E.Z.N.A.® Viral RNA Spin Protocol

The E.Z.N.A.® Mag-Bind Dye terminator Removal Procedure

关于丁香通

公司信息

个人用户

企业机构

无忧采购轻松科研

无忧采购轻松科研

提问

扫一扫

丁香实验小程序二维码

实验小助手

丁香实验公众号二维码

扫码领资料

反馈

TOP

打开小程序