• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        【求助】一个比较新的名词,因本人急用,敬请高人指教。急啊!诚恳的谢谢相助。

        丁香园论坛

        985
        A negative feedback loop exists
        whereby NF-κB drives re-synthesis of IκBα
        which promotes export of NF-κB from the
        nucleus to the cytoplasm. This process
        relies on Cezanne, a deubiquitinating
        cysteine protease that stabilizes resynthesised
        IκBα by removing
        polyubiquitin from modified
        intermediaries.这是2008年5月12日jbc上的一篇文章,文中出现Cezanne一词,我遍地搜索查询未果,应该是一个比较新的名词,因本人急用,敬请高人指教。急啊!诚恳的谢谢相助。
        以 Cezanne为题搜索Pubmed,搜到一篇题为:NF-kappaB suppression by the deubiquitinating enzyme Cezanne: A NOVEL NEGATIVE FEEDBACK LOOP IN PRO-INFLAMMATORY SIGNALING.
        PMID: 18178551
        同样也是发表在JBC 2008 Mar 14

        附摘要如下:
        Transcription factors belonging to the NF-kappaB family regulate inflammation by inducing pro-inflammatory molecules (e.g. interleukin (IL)-8) in response to cytokines (e.g. tumor necrosis factor (TNF) alpha, IL-1) or other stimuli. Several negative regulators of NF-kappaB, including the ubiquitin-editing enzyme A20, participate in the resolution of inflammatory responses. We report that Cezanne, a member of the A20 family of the deubiquitinating cysteine proteases, can be induced by TNFalpha in cultured cells. Silencing of endogenous Cezanne using small interfering RNA led to elevated NF-kappaB luciferase reporter gene activity and enhanced expression of IL-8 transcripts in TNFalpha-treated cells. Thus we conclude that endogenous Cezanne can attenuate NF-kappaB activation and the induction of pro-inflammatory transcripts in response to TNF receptor (TNFR) signaling. Overexpression studies revealed that Cezanne suppressed NF-kappaB nuclear translocation and transcriptional activity by targeting the TNFR signaling pathway at the level of the IkappaB kinase complex or upstream from it. These effects were not observed in a form of Cezanne that was mutated at the catalytic cysteine residue (Cys209), indicating that the deubiquitinating activity of Cezanne is essential for NF-kappaB regulation. Finally, we demonstrate that Cezanne can be recruited to activated TNFRs where it suppresses the build-up of polyubiquitinated RIP1 signal adapter proteins. Thus we conclude that Cezanne forms a novel negative feedback loop in pro-inflammatory signaling and that it suppresses NF-kappaB activation by targeting RIP1 signaling intermediaries for deubiquitination.

        注意红色字体,具体信号通路不是很清楚,对于专业的您或许有帮助!

        附全文链接:
        http://www.jbc.org/cgi/content/full/283/11/7036
        由衷感谢,您是做学问的人。致敬!
        您好,这个问题现在也困扰着我,请问您解决了吗?能不能给我解释一下啊?谢谢谢谢……

        本文由丁香园论坛提供,想了解更多有用的、有意思的前沿资讯以及酷炫的实验方法的你,都可以成为师兄的好伙伴

        师兄微信号:shixiongcoming

        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序