Use of Monoclonal Antibodies for Western Blotting with Enhanced Chemiluminescent Detection

Protein blotting involves the immobilization of proteins on a support medium. The most usual format is the electrophoretic transfer of proteins resolved on a sodium dodecyl sulfate (SDS)-polyacrylamide gel to a membrane, which is known as Western blotting (1 ). Polyacrylamide gel electrophoresis (PAGE) has become a standard tool wherever proteins are analyzed. Often, sufficient information may be obtained by staining the proteins in the gel. However, for applications where reaction of the protein with an antibody or ligand is required, the use of Western blots offers many advantages (2 ), the most important of which are ease of handling, improved accessibility of the proteins present, and the ability to store the immobilized proteins for further analysis.