• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Fractionation of a Microsomal Fraction from Rat Liver or Cultured Cells

        互联网

        564
        The membrane organelles of an organized tissue, such as rat liver, that will always form closed vesicles, irrespective of the mode of homogenization, are the tubular membranes of the smooth and rough endoplasmic reticulum. Almost without exception, these vesicles retain their normal orientation, i.e., the cytoplasmic face is outermost. This is particularly clear in the case of the rough endoplasmic reticulum, for the ribosomes remain attached on the outside face. The microsomal (vesicular) fraction of a homogenate will also contain vesicles derived from any domain of the plasma membrane that, in vivo, is not stabilized by structures, such as tight junctions, desmosomes, or an extensive cytoskeleton. Thus, the blood sinusoidal membrane of hepatocytes and the basolateral membranes of enterocytes both tend to form vesicles, whereas hepatocyte contiguous membranes and enterocyte microvillar membranes will remain intact, as long as the homogenization is relatively mild (see Chapter 6 ).
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序