Generation and Characterization of Ras Lipoproteins Based on Chemical Coupling
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Chemically synthesized truncated Ras proteins are coupled to C-terminal Ras peptides via a maleimidocaproyl linker. The resulting product is isolated by extraction with Triton X-114. The biological activity of these oncogenic Ras lipoproteins can be determined in a cell-based differentiation assay by microinjection into PC12 cells.
