Use of Confocal Microscopy in the Study of Microglia in a Brain Metastasis Model

Confocal imaging of brain slices is a worthwhile analysis method to study the structure and function of resting and activated microglia with submicrometer resolution. This chapter will focus on acquisition of high-resolution confocal image stacks where we will discuss the technical aspects of confocal imaging in brain sections as well as some of the currently suitable fluorescent markers for this type of work.