XL PCR Amplification of Long Targets from Genomic DNA
互联网
507
Long polymerase chain reaction (PCR)>(1 5 ), specifically, XL PCR (Extra-Long Polymerase Chain Reaction), has enabled amplification of expanded trinucleotide repeats of the neuromuscular disease myotonic dystrophy (6 ) a 9-kb HIV-1 provirus from primary isolate DNA (7 ), 24.2-kb fragments from nanogram quantities of genomic DNA for DNA damage repair (8 ), and up to 42 kb of human genomic DNA (4 ). The capability of the long PCR process stems through the use of:
