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        Silencing<br> <br> of HIF-1 by RNA Interference in Human Glioma Cells In Vitro and In Vivo

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        Higher-grade gliomas are distinguished by increased vascular endothelial cell proliferation and peritumoral edema. These are thought to be instigated by vascular endothelial growth factor, which in turn is regulated by cellular oxygen tension. Hypoxia inducible factor-1α (HIF-1α) is a main responder to intracellular hypoxia and is overexpressed in many human cancers, including gliomas. Here we present methods for investigating the role of HIF-1α in glioma growth in vivo and in vitro using RNA interference in U251, U87, and U373 glioma cells.
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