Enriching Basic and Acidic Rat Brain Proteins with Ion<br>Exchange Mini Spin Columns Before Two-Dimensional<br>Gel Electrophoresis

Proteome analysis by two-dimensional gel electrophoresis (2-DGE) faces significant challenges because of the complexity of biological samples. However, the complexity of a protein sample can be reduced prior to 2-DGE by applying protein fractionation. Protein fractionation allows analysis of one protein subset at a time, thereby, increasing the load of proteins of interest, enriching low-abundance proteins, and increasing the resolution of protein spots on a 2-D gel.