• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Site-Directed DNA Photoaffinity Labeling of RNA Polymerase III Transcription Complexes

        互联网

        585
        Site-specific DNA photoaffinity labeling is a useful technique for mapping interactions of proteins with DNA in complex systems such as the yeast RNA polymerase III (Pol III) transcription complex, which consists of at least 25 different proteins (1 ,2 ). This technique allows probing of protein-DNA interactions across large stretches of DNA and can be done in relatively crude extracts. The regions or domains of the protein contacting DNA can be identified by peptide mapping of the photoaffinity labeled protein. Our discussion of DNA photoaffinity labeling will focus on (1 ) the synthesis of photoreactive nucleotide analogs, (2 ) the manner in which the photoreactive nucleotide is incorporated into DNA, and (3 ) experimental details of DNA photoaffinity labeling.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序